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Literature summary extracted from

  • Brown-Petersen, N.J.; Salin, M.L.
    Purification of a catalase-peroxidase from Halobacterium halobium: Characterization of some unique properties of the halophilic enzyme (1993), J. Bacteriol., 175, 4197-4202.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.11.1.6 NaCl highest activity for catalase at 2 M, for peroxidase at 1 M Halobacterium salinarum
1.11.1.21 NaCl peroxidase activity is maximal at NaCl concentrations above 1 M, although catalase activity requires 2 M NaCl for optimal function Halobacterium salinarum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.11.1.6 3-amino-1H-1,2,4-triazole not inhibitory Halobacterium salinarum
1.11.1.6 H2O2 50% inhibition for catalase at 4.5 mM, for peroxidase at 0.4 mM Halobacterium salinarum
1.11.1.6 KCN effective inhibition Halobacterium salinarum
1.11.1.6 NaN3 effective inhibition Halobacterium salinarum
1.11.1.21 H2O2
-
 Halobacterium salinarum
1.11.1.21 KCN catalase and peroxidase activities are effectively inhibited by KCN Halobacterium salinarum
1.11.1.21 additional information catalatic activity of the purified H catalase-peroxidase is not inhibited by incubation for 2.5 h with 20 mM 3 amino-1,2,4-triazole Halobacterium salinarum
1.11.1.21 NaN3 catalase and peroxidase activities are effectively inhibited by NaN3 Halobacterium salinarum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.6 0.14
-
 H2O2 peroxidase activity Halobacterium salinarum
1.11.1.6 3.7
-
 H2O2 catalase activity Halobacterium salinarum
1.11.1.21 0.14
-
 H2O2 peroxidase activity, in 50 mM potassium phosphate containing 2 M NaCl, pH 7.5, at 25°C Halobacterium salinarum
1.11.1.21 3.7
-
 H2O2 catalase activity, in 50 mM potassium phosphate containing 2 M NaCl, pH 6.5, at 25°C Halobacterium salinarum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.6 60300
-
 4 * 60300, SDS-PAGE Halobacterium salinarum
1.11.1.6 240000
-
 gel filtration Halobacterium salinarum
1.11.1.21 60300
-
 4 * 60300, estimated from SDS-PAGE Halobacterium salinarum
1.11.1.21 240000
-
 gel filtration Halobacterium salinarum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.11.1.6 H2O2 Halobacterium salinarum bifunctional catalase-peroxidase O2 + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.6 Halobacterium salinarum
-
-
-
1.11.1.21 Halobacterium salinarum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.6 to homogeneity, chromatography steps Halobacterium salinarum
1.11.1.21 Macrosphere GPC 300 column chromatography, hydroxylapatite column chromatography, and Sephadex G-150 gel filtration Halobacterium salinarum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.11.1.6 cell culture
-
 Halobacterium salinarum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.11.1.6 43.2
-
 catalase activity Halobacterium salinarum
1.11.1.6 104.3
-
 peroxidase activity Halobacterium salinarum
1.11.1.21 3.4
-
 crude extract, catalase activity, in 50 mM potassium phosphate containing 2 M NaCl, pH 6.5, at 25°C Halobacterium salinarum
1.11.1.21 3.9
-
 crude extract, peroxidase activity, in 50 mM potassium phosphate containing 2 M NaCl, pH 7.5, at 25°C Halobacterium salinarum
1.11.1.21 43.2
-
 after 12.7fold purification, catalase activity, in 50 mM potassium phosphate containing 2 M NaCl, pH 6.5, at 25°C Halobacterium salinarum
1.11.1.21 104.3
-
 after 29.6fold purification, peroxidase activity, in 50 mM potassium phosphate containing 2 M NaCl, pH 7.5, at 25°C Halobacterium salinarum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.6 3,3'-diaminobenzidine + H2O2
-
 Halobacterium salinarum ? + H2O
-
 ?
1.11.1.6 4-aminoantipyrine + H2O2
-
 Halobacterium salinarum ? + H2O
-
 ?
1.11.1.6 ascorbate + H2O2
-
 Halobacterium salinarum ? + H2O
-
 ?
1.11.1.6 H2O2 bifunctional catalase-peroxidase Halobacterium salinarum O2 + H2O
-
 ?
1.11.1.6 NADH + H2O2
-
 Halobacterium salinarum NAD+ + H2O
-
 ?
1.11.1.6 NADPH + H2O2
-
 Halobacterium salinarum NADP+ + H2O
-
 ?
1.11.1.6 o-dianisidine + H2O2
-
 Halobacterium salinarum ? + H2O
-
 ?
1.11.1.6 reduced cytochrome c + H2O2
-
 Halobacterium salinarum oxidized cytochrome c + H2O
-
 ?
1.11.1.21 3,3'-diaminobenzidine + H2O2
-
 Halobacterium salinarum oxidized 3,3'-diaminobenzidine + H2O
-
 ?
1.11.1.21 4-aminoantipyrine + H2O2
-
 Halobacterium salinarum oxidized 4-aminoantipyrine + H2O
-
 ?
1.11.1.21 ascorbate + H2O2
-
 Halobacterium salinarum monodehydroascorbate + H2O
-
 ?
1.11.1.21 H2O2
-
 Halobacterium salinarum O2 + H2O
-
 ?
1.11.1.21 NADH + H2O2
-
 Halobacterium salinarum ?
-
 ?
1.11.1.21 NADPH + H2O2
-
 Halobacterium salinarum ?
-
 ?
1.11.1.21 o-dianisidine + H2O2
-
 Halobacterium salinarum oxidized o-dianisidine + H2O
-
 ?
1.11.1.21 reduced cytochrome c + H2O2
-
 Halobacterium salinarum oxidized cytochrome c + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.11.1.6 tetramer 4 * 60300, SDS-PAGE Halobacterium salinarum
1.11.1.21 homotetramer 4 * 60300, estimated from SDS-PAGE Halobacterium salinarum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.11.1.6 40
-
 peroxidase activity Halobacterium salinarum
1.11.1.6 50
-
 catalase activity Halobacterium salinarum
1.11.1.21 40
-
 peroxidase activity decreases rapidly above its maximum at 40°C Halobacterium salinarum
1.11.1.21 50
-
 catalase activity Halobacterium salinarum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.11.1.6 70
-
 50% of activity retained for peroxidase activity Halobacterium salinarum
1.11.1.6 90
-
 catalase activity stable up to Halobacterium salinarum
1.11.1.21 25 90 at 90°C, the catalatic activity is 20% greater than at 25°C. Peroxidatic activity declines after incubation at temperatures higher than 40°C, although at 70°C nearly 50% of the activity at 25°C is retained Halobacterium salinarum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.6 6.5
-
 catalase activity Halobacterium salinarum
1.11.1.6 7.5
-
 peroxidase activity Halobacterium salinarum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.11.1.21 6 7.5 catalase activity Halobacterium salinarum
1.11.1.21 6.5 8 peroxidase activity Halobacterium salinarum

Cofactor

EC Number Cofactor Comment Organism Structure
1.11.1.6 heme 1.43 hemes per tetramer Halobacterium salinarum
1.11.1.21 heme heme content of 1.43 per tetramer Halobacterium salinarum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.11.1.6 0.03
-
 NaN3
-
 Halobacterium salinarum
1.11.1.6 0.05
-
 KCN
-
 Halobacterium salinarum
1.11.1.21 0.03
-
 NaN3 apparent value, peroxidase activity, in 50 mM potassium phosphate containing 2 M NaCl, pH 7.5, at 25°C Halobacterium salinarum
1.11.1.21 0.05
-
 KCN apparent value, catalase activity, in 50 mM potassium phosphate containing 2 M NaCl, pH 6.5, at 25°C Halobacterium salinarum
1.11.1.21 0.05
-
 KCN apparent value, peroxidase activity, in 50 mM potassium phosphate containing 2 M NaCl, pH 7.5, at 25°C Halobacterium salinarum
1.11.1.21 0.0675
-
 NaN3 apparent value, catalase activity, in 50 mM potassium phosphate containing 2 M NaCl, pH 6.5, at 25°C Halobacterium salinarum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.11.1.21 Halobacterium salinarum isoelectric focusing
-
 3.8

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.11.1.21 0.4
-
 peroxidase activity, in 50 mM potassium phosphate containing 2 M NaCl, pH 7.5, at 25°C Halobacterium salinarum H2O2
1.11.1.21 4.5
-
 catalase activity, in 50 mM potassium phosphate containing 2 M NaCl, pH 6.5, at 25°C Halobacterium salinarum H2O2