BRENDA - Enzyme Database

Purification and characterization of catalase from goat (Capra capra) lung

Chatterjee, U.; Sanwal, G.G.; Mol. Cell. Biochem. 126, 125-133 (1993)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.11.1.6
2-mercaptoethanol
complete inhibition at 23 mM, summation effect with p-chloromercuribenzoate
Capra capra
1.11.1.6
5,5-dithiobis(2-nitrobenzoic acid)
complete inhibition at 0.4 mM
Capra capra
1.11.1.6
BO3-
weak inhibition at 1 mM
Capra capra
1.11.1.6
Cu2+
55% inhibition at 0.2 mM
Capra capra
1.11.1.6
dithiothreitol
complete inhibition at 33 mM
Capra capra
1.11.1.6
formaldehyde
non-competitive inhibition
Capra capra
1.11.1.6
indole acetic acid
non-competitive inhibition
Capra capra
1.11.1.6
iodoacetamide
complete inhibition at 11.7 mM
Capra capra
1.11.1.6
L-aspartic acid
25% inhibition at 16 mM
Capra capra
1.11.1.6
L-cysteine
35% inhibition at 3.3 mM
Capra capra
1.11.1.6
L-lactic acid
63% inhibition at 25 mM
Capra capra
1.11.1.6
L-Malic acid
50% inhibition at 6 mM
Capra capra
1.11.1.6
L-tryptophan
80% inhibition at 1.6 mM
Capra capra
1.11.1.6
L-tyrosine
80% inhibition at 5 mM
Capra capra
1.11.1.6
Maleic acid
51% inhibition at 0.8 mM
Capra capra
1.11.1.6
Mn2+
30% inhibition at 1 mM
Capra capra
1.11.1.6
N-bromosuccinimide
complete inhibition at 3.34 mM
Capra capra
1.11.1.6
N-ethylmaleimide
complete inhibition at 18.3 mM
Capra capra
1.11.1.6
NaN3
non-competitive inhibition
Capra capra
1.11.1.6
oxalic acid
51% inhibition at 4 mM
Capra capra
1.11.1.6
p-chloromercuribenzoate
complete inhibition at 0.33 mM, summation effect with 2-mercaptoethanol
Capra capra
1.11.1.6
Pyruvic acid
16% inhibition at 5 mM
Capra capra
1.11.1.6
SO3-
weak inhibition at 4 mM
Capra capra
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.11.1.6
70
-
H2O2
-
Capra capra
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.11.1.6
339000
-
gel filtration
Capra capra
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.11.1.6
H2O2
Capra capra
-
O2 + H2O
-
Capra capra
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.11.1.6
Capra capra
-
goat
-
Purification (Commentary)
EC Number
Commentary
Organism
1.11.1.6
to homogeneity, chromatography techniques
Capra capra
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.11.1.6
lung
-
Capra capra
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.11.1.6
38330
-
-
Capra capra
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.11.1.6
H2O2
-
439788
Capra capra
O2 + H2O
-
439788
Capra capra
?
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.11.1.6
217000
-
H2O2
-
Capra capra
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.11.1.6
5.2
7.8
-
Capra capra
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.11.1.6
0.0017
-
NaN3
non-competitive inhibition
Capra capra
1.11.1.6
0.55
-
formaldehyde
non-competitive inhibition
Capra capra
1.11.1.6
1.5
-
L-tryptophan
non-competitive inhibition
Capra capra
1.11.1.6
1.6
-
indole acetic acid
non-competitive inhibition
Capra capra
1.11.1.6
6.7
-
L-cysteine
non-competitive inhibition
Capra capra
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.11.1.6
2-mercaptoethanol
complete inhibition at 23 mM, summation effect with p-chloromercuribenzoate
Capra capra
1.11.1.6
5,5-dithiobis(2-nitrobenzoic acid)
complete inhibition at 0.4 mM
Capra capra
1.11.1.6
BO3-
weak inhibition at 1 mM
Capra capra
1.11.1.6
Cu2+
55% inhibition at 0.2 mM
Capra capra
1.11.1.6
dithiothreitol
complete inhibition at 33 mM
Capra capra
1.11.1.6
formaldehyde
non-competitive inhibition
Capra capra
1.11.1.6
indole acetic acid
non-competitive inhibition
Capra capra
1.11.1.6
iodoacetamide
complete inhibition at 11.7 mM
Capra capra
1.11.1.6
L-aspartic acid
25% inhibition at 16 mM
Capra capra
1.11.1.6
L-cysteine
35% inhibition at 3.3 mM
Capra capra
1.11.1.6
L-lactic acid
63% inhibition at 25 mM
Capra capra
1.11.1.6
L-Malic acid
50% inhibition at 6 mM
Capra capra
1.11.1.6
L-tryptophan
80% inhibition at 1.6 mM
Capra capra
1.11.1.6
L-tyrosine
80% inhibition at 5 mM
Capra capra
1.11.1.6
Maleic acid
51% inhibition at 0.8 mM
Capra capra
1.11.1.6
Mn2+
30% inhibition at 1 mM
Capra capra
1.11.1.6
N-bromosuccinimide
complete inhibition at 3.34 mM
Capra capra
1.11.1.6
N-ethylmaleimide
complete inhibition at 18.3 mM
Capra capra
1.11.1.6
NaN3
non-competitive inhibition
Capra capra
1.11.1.6
oxalic acid
51% inhibition at 4 mM
Capra capra
1.11.1.6
p-chloromercuribenzoate
complete inhibition at 0.33 mM, summation effect with 2-mercaptoethanol
Capra capra
1.11.1.6
Pyruvic acid
16% inhibition at 5 mM
Capra capra
1.11.1.6
SO3-
weak inhibition at 4 mM
Capra capra
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.11.1.6
0.0017
-
NaN3
non-competitive inhibition
Capra capra
1.11.1.6
0.55
-
formaldehyde
non-competitive inhibition
Capra capra
1.11.1.6
1.5
-
L-tryptophan
non-competitive inhibition
Capra capra
1.11.1.6
1.6
-
indole acetic acid
non-competitive inhibition
Capra capra
1.11.1.6
6.7
-
L-cysteine
non-competitive inhibition
Capra capra
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.11.1.6
70
-
H2O2
-
Capra capra
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.11.1.6
339000
-
gel filtration
Capra capra
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.11.1.6
H2O2
Capra capra
-
O2 + H2O
-
Capra capra
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.11.1.6
to homogeneity, chromatography techniques
Capra capra
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.11.1.6
lung
-
Capra capra
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.11.1.6
38330
-
-
Capra capra
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.11.1.6
H2O2
-
439788
Capra capra
O2 + H2O
-
439788
Capra capra
?
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.11.1.6
217000
-
H2O2
-
Capra capra
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.11.1.6
5.2
7.8
-
Capra capra