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Literature summary extracted from

  • Fraaije, M.W.; Roubroeks, H.P.; Hagen, W.R.; Van Berkel, W.J.
    Purification and characterization of an intracellular catalase-peroxidase from Penicillium simplicissimum (1996), Eur. J. Biochem., 235, 192-198.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.11.1.6 3-amino-1H-1,2,4-triazole no effect on catalase-peroxidase enzyme Penicillium simplicissimum
1.11.1.6 KCN
-
Penicillium simplicissimum
1.11.1.6 NaN3
-
Penicillium simplicissimum
1.11.1.21 azide
-
Penicillium simplicissimum
1.11.1.21 cyanide
-
Penicillium simplicissimum
1.11.1.21 additional information incubation for 60 min with 20 mM 3-amino-l,2,4-triazole has no effect on enzyme activity Penicillium simplicissimum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.21 10.8
-
H2O2 catalase activity, at pH 6.4 and 25°C Penicillium simplicissimum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.11.1.6 intracellular catalase-peroxidase enzyme Penicillium simplicissimum 5622
-
1.11.1.6 periplasm catalase only Penicillium simplicissimum
-
-
1.11.1.21 protoplast
-
Penicillium simplicissimum
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.6 85000
-
catalase-peroxidase, 2 * 85000, SDS-PAGE Penicillium simplicissimum
1.11.1.6 170000
-
catalase-peroxidase Penicillium simplicissimum
1.11.1.21 83000
-
2 * 83000, SDS-PAGE Penicillium simplicissimum
1.11.1.21 170000
-
gel filtration Penicillium simplicissimum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.11.1.6 H2O2 Penicillium simplicissimum one catalase-peroxidase, one catalase enzyme O2 + H2O
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.6 Penicillium simplicissimum
-
-
-
1.11.1.21 Penicillium simplicissimum
-
-
-
1.11.1.21 Penicillium simplicissimum CBS 170.90
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.11.1.21 additional information PCP does not contain a significant amount of carbohydrate Penicillium simplicissimum

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.6 catalase-peroxidase enzyme to homogeneity, catalase enzyme partial Penicillium simplicissimum
1.11.1.21 ammonium sulfate precipitation, phenyl-Sepharose column chromatography, Q-Sepharose column chromatography, hydroxyapatite column chromatography, and Superdex PG-200 gel filtration Penicillium simplicissimum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.11.1.6 cell culture
-
Penicillium simplicissimum
-
1.11.1.21 culture condition:veratryl alcohol-grown cell
-
Penicillium simplicissimum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.11.1.21 7.5
-
peroxidase activity using guaicol as substrate, at pH 5.4 and 25°C Penicillium simplicissimum
1.11.1.21 16
-
peroxidase activity using 3,3'-diaminobenzidine as substrate, at pH 5.4 and 25°C Penicillium simplicissimum
1.11.1.21 28
-
peroxidase activity using 3,3'-dimethoxybenzidine as substrate, at pH 5.4 and 25°C Penicillium simplicissimum
1.11.1.21 77
-
peroxidase activity using 2,6-dimethoxyphenol as substrate, at pH 5.4 and 25°C Penicillium simplicissimum
1.11.1.21 150
-
peroxidase activity using 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) as substrate, at pH 5.4 and 25°C Penicillium simplicissimum
1.11.1.21 250
-
cell extract, at 25°C, pH 6.4 Penicillium simplicissimum
1.11.1.21 2960
-
purified enzyme, at 25°C, pH 6.4 Penicillium simplicissimum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.6 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2
-
Penicillium simplicissimum ? + H2O
-
?
1.11.1.6 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2
-
Penicillium simplicissimum CBS 170.90 ? + H2O
-
?
1.11.1.6 2,6-dimethoxyphenol + H2O2
-
Penicillium simplicissimum ? + H2O
-
?
1.11.1.6 2,6-dimethoxyphenol + H2O2
-
Penicillium simplicissimum CBS 170.90 ? + H2O
-
?
1.11.1.6 3,3'-diaminobenzidine + H2O2
-
Penicillium simplicissimum ? + H2O
-
?
1.11.1.6 3,3'-diaminobenzidine + H2O2
-
Penicillium simplicissimum CBS 170.90 ? + H2O
-
?
1.11.1.6 3,3'-dimethoxybenzidine + H2O2
-
Penicillium simplicissimum ? + H2O
-
?
1.11.1.6 3,3'-dimethoxybenzidine + H2O2
-
Penicillium simplicissimum CBS 170.90 ? + H2O
-
?
1.11.1.6 H2O2 one catalase-peroxidase, one catalase enzyme Penicillium simplicissimum O2 + H2O
-
?
1.11.1.6 o-methoxyphenol + H2O2 i.e. guaiacol Penicillium simplicissimum ? + H2O
-
?
1.11.1.6 o-methoxyphenol + H2O2 i.e. guaiacol Penicillium simplicissimum CBS 170.90 ? + H2O
-
?
1.11.1.21 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2
-
Penicillium simplicissimum oxidized 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O
-
?
1.11.1.21 2,6-dimethoxyphenol + H2O2
-
Penicillium simplicissimum oxidized 2,6-dimethoxyphenol + H2O
-
?
1.11.1.21 3,3'-diaminobenzidine + H2O2
-
Penicillium simplicissimum oxidized 3,3'-diaminobenzidine + H2O
-
?
1.11.1.21 3,3'-dimethoxybenzidine + H2O2
-
Penicillium simplicissimum oxidized 3,3'-dimethoxybenzidine + H2O
-
?
1.11.1.21 guaiacol + H2O2
-
Penicillium simplicissimum tetraguaiacol + H2O
-
?
1.11.1.21 H2O2
-
Penicillium simplicissimum O2 + H2O
-
?
1.11.1.21 additional information NADPH, vanilly1 alcohol and veratryl alcohol do not serve as electron donors Penicillium simplicissimum ?
-
?

Subunits

EC Number Subunits Comment Organism
1.11.1.6 dimer catalase-peroxidase, 2 * 85000, SDS-PAGE Penicillium simplicissimum
1.11.1.21 homodimer 2 * 83000, SDS-PAGE Penicillium simplicissimum

Synonyms

EC Number Synonyms Comment Organism
1.11.1.21 PCP
-
Penicillium simplicissimum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.11.1.21 25
-
the enzyme remains fully active at pH 5.0 for more than 15 min at 25°C Penicillium simplicissimum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.6 6.4
-
catalase-peroxidase enzyme Penicillium simplicissimum
1.11.1.21 5.4
-
pH optimum for peroxidase activity Penicillium simplicissimum
1.11.1.21 6.4
-
pH optimum for catalase activity Penicillium simplicissimum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.11.1.21 5 9 at pH 5.0 all activity is lost within 5 min whereas no inactivation is observed between pH 7.0 and pH 9.0 at this temperature after a 10 h incubation Penicillium simplicissimum

Cofactor

EC Number Cofactor Comment Organism Structure
1.11.1.6 heme the monofunctional catalase contains a chlorine-type heme, the catalase-peroxidase has protoheme IX Penicillium simplicissimum
1.11.1.21 heme
-
Penicillium simplicissimum

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.11.1.21 0.003
-
at 25°C, pH 6.4 Penicillium simplicissimum cyanide
1.11.1.21 0.017
-
at 25°C, pH 6.4 Penicillium simplicissimum azide