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Literature summary extracted from

  • Shin, K.S.; Oh, I.K.; Kim, C.J.
    Production and purification of Remazol Brilliant Blue R decolorizing peroxidase from the culture filtrate of Pleurotus ostreatus (1997), Appl. Environ. Microbiol., 63, 1744-1748.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.11.1.7 KCN 76% inhibition at 0.1 mM Pleurotus ostreatus
1.11.1.7 L-cysteine 94% inhibition at 0.1 mM Pleurotus ostreatus
1.11.1.7 Na2S2O5 100% inhibition at 0.1 mM Pleurotus ostreatus
1.11.1.7 NaN3 95% inhibition at 0.1 mM Pleurotus ostreatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.7 0.006
-
 Crystal violet
-
 Pleurotus ostreatus
1.11.1.7 0.011
-
 Remazol Brilliant Blue R
-
 Pleurotus ostreatus
1.11.1.7 0.017
-
 Phenol red
-
 Pleurotus ostreatus
1.11.1.7 0.021
-
 sinapinic acid
-
 Pleurotus ostreatus
1.11.1.7 0.028
-
 syringic acid
-
 Pleurotus ostreatus
1.11.1.7 0.029
-
 malachite green
-
 Pleurotus ostreatus
1.11.1.7 0.033
-
 H2O2
-
 Pleurotus ostreatus
1.11.1.7 0.054
-
 vanillic acid
-
 Pleurotus ostreatus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.11.1.7 extracellular
-
 Pleurotus ostreatus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.7 71000
-
 1 * 71000, SDS-PAGE Pleurotus ostreatus
1.11.1.7 73000
-
 gel filtration Pleurotus ostreatus

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.7 Pleurotus ostreatus
-
-
-
1.11.1.7 Pleurotus ostreatus NFFA
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.11.1.7 glycoprotein 38% carbohydrate by weight Pleurotus ostreatus

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.7
-
 Pleurotus ostreatus

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.11.1.7 6
-
 with Remazol Brilliant Blue R Pleurotus ostreatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.7 crystal violet + H2O2
-
 Pleurotus ostreatus ?
-
 ?
1.11.1.7 crystal violet + H2O2
-
 Pleurotus ostreatus NFFA ?
-
 ?
1.11.1.7 malachite green + H2O2
-
 Pleurotus ostreatus ?
-
 ?
1.11.1.7 phenol red + H2O2
-
 Pleurotus ostreatus ?
-
 ?
1.11.1.7 phenol red + H2O2
-
 Pleurotus ostreatus NFFA ?
-
 ?
1.11.1.7 Remazol Brilliant Blue R + H2O2
-
 Pleurotus ostreatus ?
-
 ?
1.11.1.7 Remazol Brilliant Blue R + H2O2
-
 Pleurotus ostreatus NFFA ?
-
 ?
1.11.1.7 sinapic acid + H2O2
-
 Pleurotus ostreatus ?
-
 ?
1.11.1.7 sinapic acid + H2O2
-
 Pleurotus ostreatus NFFA ?
-
 ?
1.11.1.7 syringic acid + H2O2
-
 Pleurotus ostreatus ?
-
 ?
1.11.1.7 syringic acid + H2O2
-
 Pleurotus ostreatus NFFA ?
-
 ?
1.11.1.7 vanillic acid + H2O2
-
 Pleurotus ostreatus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.11.1.7 monomer 1 * 71000, SDS-PAGE Pleurotus ostreatus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.11.1.7 30
-
 stable at pH 4-6 for 1 h Pleurotus ostreatus
1.11.1.7 70
-
 complete inactivation Pleurotus ostreatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.7 3 3.5 at 25°C Pleurotus ostreatus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.11.1.7 4 6 stable at 30°C for 1 h Pleurotus ostreatus