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Literature summary extracted from

  • Schneider, K.; Cammack, R.; Schlegel, H.G.
    Content and localization of FMN, Fe-S clusters and nickel in the NAD-linked hydrogenase of Nocardia opaca 1b (1984), Eur. J. Biochem., 142, 75-84.
    View publication on PubMed

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.12.1.2 Iron non-heme iron protein Rhodococcus opacus
1.12.1.2 Iron 13.6 iron atoms per enzyme molecule Rhodococcus opacus
1.12.1.2 Ni2+ nickel protein Rhodococcus opacus
1.12.1.2 Ni2+ 3.8 nickel atoms per enzyme molecule Rhodococcus opacus

Organism

EC Number Organism UniProt Comment Textmining
1.12.1.2 Rhodococcus opacus
-
-
-
1.12.1.2 Rhodococcus opacus 1B
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.12.1.2 H2 + NAD+
-
 Rhodococcus opacus H+ + NADH
-
 ?
1.12.1.2 H2 + NAD+
-
 Rhodococcus opacus 1B H+ + NADH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.12.1.2 More large dimer composed of 64000 Da and 31000 Da subunit contains 1 FMN, 1 [2Fe-2S] and 2 [4Fe-4S] cluster, diaphorase activity is located on large dimer, small dimer composed of 56000 Da and 27000 Da subunits contains 2 Ni and 1 [4Fe-4S]/[3Fe-xS] cluster, hydrogenase activity is localized on small dimer Rhodococcus opacus

Cofactor

EC Number Cofactor Comment Organism Structure
1.12.1.2 flavin flavoprotein Rhodococcus opacus
1.12.1.2 flavin 1 molecule of FMN per enzyme molecule Rhodococcus opacus
1.12.1.2 FMN 1 FMN molecule per enzyme molecule Rhodococcus opacus