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Literature summary extracted from

  • Florin, L.; Tsokoglou, A.; Happe, T.
    A novel type of iron hydrogenase in the green alga Scenedesmus obliquus is linked to the photosynthetic electron transport chain (2001), J. Biol. Chem., 276, 6125-6132.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.12.7.2 expressed in Escherichia coli Tetradesmus obliquus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.12.7.2 2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone inhibition of H2 production, photosystem 1 is involved in the supply of electrons to the hydrogenase Tetradesmus obliquus
1.12.7.2 CO strong inhibition Tetradesmus obliquus
1.12.7.2 O2 strong inhibition Tetradesmus obliquus
1.12.7.2 sulfo-disalicylidinepropandiamine hydrogenase activity is reduced up to 30-fold Tetradesmus obliquus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.12.7.2 0.8
-
 methyl viologen
-
 Tetradesmus obliquus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.12.7.2 chloroplast
-
 Tetradesmus obliquus 9507
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.12.7.2 Fe the catalytic site is an H-cluster, containing a [4Fe4S] subcluster and a [2Fe] center Tetradesmus obliquus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.12.7.2 44000
-
 1 * 44000, SDS-PAGE Tetradesmus obliquus
1.12.7.2 44500
-
 gel filtration and SDS-PAGE Tetradesmus obliquus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.12.7.2 H2 + oxidized ferredoxin Tetradesmus obliquus ferredoxin links the enzyme to photosynthesis H+ + reduced ferredoxin
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.12.7.2 Tetradesmus obliquus
-
 wild-type Kützing 276-6
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.12.7.2 rapid inactivation under aerobic conditions Tetradesmus obliquus

Purification (Commentary)

EC Number Purification (Comment) Organism
1.12.7.2 strictly under anaerobic conditions Tetradesmus obliquus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.12.7.2 700
-
 hydrogen evolution Tetradesmus obliquus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.12.7.2 H2 + electron acceptor methyl viologen as electron acceptor Tetradesmus obliquus H+ + reduced electron acceptor
-
 r
1.12.7.2 H2 + oxidized ferredoxin ferredoxin links the enzyme to photosynthesis Tetradesmus obliquus H+ + reduced ferredoxin
-
 r
1.12.7.2 H2 + oxidized methyl viologen
-
 Tetradesmus obliquus H+ + reduced methyl viologen
-
 r

Subunits

EC Number Subunits Comment Organism
1.12.7.2 monomer 1 * 44000, SDS-PAGE Tetradesmus obliquus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.12.7.2 60
-
-
 Tetradesmus obliquus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.12.7.2 7.3
-
-
 Tetradesmus obliquus