Literature summary extracted from

Czechowski, M.H.; He, S.H.; Nacro, M.; DerVartanian, D.V.; Peck, H.D., Jr.; LeGall, J.
A cytoplasmic nickel-iron hydrogenase with high specific activity from Desulfovibrio multispirans sp. n., a new species of sulfate reducing bacterium (1984), Biochem. Biophys. Res. Commun., 125, 1025-1032.

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.12.99.6	0.41	-	H2	with methyl viologen as electron acceptor	Desulfovibrio multispirans

Localization

EC Number	Localization	Comment	Organism	GeneOntology No.	Textmining
1.12.99.6	cytoplasm	-	Desulfovibrio multispirans	5737	-

Metals/Ions

EC Number	Metals/Ions	Comment	Organism	Structure
1.12.99.6	Fe	11 atoms per mol enzyme	Desulfovibrio multispirans
1.12.99.6	Ni	0.9 atoms per mol enzyme	Desulfovibrio multispirans
1.12.99.6	Sulfide	10 atoms labile sulfide per mol enzyme	Desulfovibrio multispirans

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
1.12.99.6	24500	-	1 * 58000 + 1 * 24500, SDS-PAGE	Desulfovibrio multispirans
1.12.99.6	58000	-	1 * 58000 + 1 * 24500, SDS-PAGE	Desulfovibrio multispirans
1.12.99.6	82000	-	gel filtration	Desulfovibrio multispirans

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.12.99.6	Desulfovibrio multispirans	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
1.12.99.6	apparent homogeneity	Desulfovibrio multispirans

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1.12.99.6	792	-	-	Desulfovibrio multispirans

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.12.99.6	H2 + acceptor	enzyme is more active in hydrogen evolution than in hydrogen uptake	Desulfovibrio multispirans	H+ + reduced acceptor	-	?

Subunits

EC Number	Subunits	Comment	Organism
1.12.99.6	dimer	1 * 58000 + 1 * 24500, SDS-PAGE	Desulfovibrio multispirans

Temperature Optimum [°C]

EC Number	Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
1.12.99.6	70	-	-	Desulfovibrio multispirans

Temperature Stability [°C]

EC Number	Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
1.12.99.6	50	-	stable for 60 min	Desulfovibrio multispirans
1.12.99.6	70	-	stable for 20 min	Desulfovibrio multispirans

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Release 2023.1 (January 2023)