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Literature summary extracted from

  • Suzuki, I.; Silver, M.
    The initial product and properties of the sulfur-oxidizing enzyme of thiobacilli (1966), Biochim. Biophys. Acta, 122, 22-33.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.13.11.18 1,10-phenanthroline similar stimulating effect as 2,2' dipyridyl Thiobacillus thioparus
1.13.11.18 2,2'-dipyridyl stimulation at low concentrations e.g. 0.1 mM, may protect glutathione from destruction Thiobacillus thioparus
1.13.11.18 catalase
-
 Thiobacillus thioparus

General Stability

EC Number General Stability Organism
1.13.11.18 2,2'-dipyridyl stabilizes cofactor glutathione, activity is lost upon freezing and thawing Thiobacillus thioparus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.18 Co2+ 0.1 mM Thiobacillus thioparus
1.13.11.18 Cu2+ 0.1 mM Thiobacillus thioparus
1.13.11.18 diethyldithiocarbamate
-
 Thiobacillus thioparus
1.13.11.18 EDTA
-
 Thiobacillus thioparus
1.13.11.18 FAD
-
 Thiobacillus thioparus
1.13.11.18 Fe2+
-
 Thiobacillus thioparus
1.13.11.18 Fe3+
-
 Thiobacillus thioparus
1.13.11.18 FMN
-
 Thiobacillus thioparus
1.13.11.18 H2O2 strong inhibition, reversible by subsequent addition of catalase and glutathione Thiobacillus thioparus
1.13.11.18 Zn2+ 0.1 mM Thiobacillus thioparus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.18 6.3
-
 glutathione
-
 Thiobacillus thioparus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.18 non-heme iron
-
 Acidithiobacillus thiooxidans
1.13.11.18 non-heme iron 0.000087 mmol of iron per mg of enzyme Thiobacillus thioparus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.18 sulfur + O2 + H2O Thiobacillus thioparus
-
 sulfite
-
 ir
1.13.11.18 sulfur + O2 + H2O Acidithiobacillus thiooxidans
-
 sulfite
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.18 Acidithiobacillus thiooxidans
-
-
-
1.13.11.18 Thiobacillus thioparus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.18 30% ethanol, DEAE-cellulose Thiobacillus thioparus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.18 0.25
-
-
 Thiobacillus thioparus

Storage Stability

EC Number Storage Stability Organism
1.13.11.18 -20°C, DEAE-cellulose fraction, 7 d, almost complete loss of activity Thiobacillus thioparus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.18 glutathione + sulfite + 2 H+
-
 Thiobacillus thioparus S-sulfanylglutathione + O2 + H2O
-
 r
1.13.11.18 sulfur + O2 + H2O
-
 Thiobacillus thioparus sulfite
-
 ir
1.13.11.18 sulfur + O2 + H2O
-
 Acidithiobacillus thiooxidans sulfite
-
 ir
1.13.11.18 sulfur + O2 + H2O
-
 Thiobacillus thioparus sulfite S2O32- may be formed through a secondary, non-enzymatic reaction ir
1.13.11.18 sulfur + O2 + H2O
-
 Acidithiobacillus thiooxidans sulfite S2O32- may be formed through a secondary, non-enzymatic reaction ir

Cofactor

EC Number Cofactor Comment Organism Structure
1.13.11.18 glutathione
-
 Thiobacillus thioparus
1.13.11.18 glutathione
-
 Acidithiobacillus thiooxidans