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Literature summary extracted from

  • Kletzin, A.
    Coupled enzymatic production of sulfite, thiosulfate, and hydrogen sulfide from sulfur: purification and properties of a sulfur oxygenase reductase from the facultatively anaerobic archaebacterium Desulfurolobus ambivalens (1989), J. Bacteriol., 171, 1638-1643.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
1.13.11.55 loses more than 80% of activity after three freezing and thawing steps Acidianus ambivalens
1.13.11.55 partially inactivated by freezing at -80°C Acidianus ambivalens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.55 2-iodoacetic acid
-
Acidianus ambivalens
1.13.11.55 4-chloromercuribenzoic acid
-
Acidianus ambivalens
1.13.11.55 FAD
-
Acidianus ambivalens
1.13.11.55 Fe2+
-
Acidianus ambivalens
1.13.11.55 Fe3+
-
Acidianus ambivalens
1.13.11.55 additional information the enmzyme is not affected by CN-, N3-, or reduced glutathione Acidianus ambivalens
1.13.11.55 N-ethylmaleimide
-
Acidianus ambivalens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.13.11.55 cytoplasm
-
Acidianus ambivalens 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.55 40000
-
x * 40000, SDS-PAGE Acidianus ambivalens
1.13.11.55 550000
-
gel filtration Acidianus ambivalens

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.55 Acidianus ambivalens P29082
-
-
1.13.11.55 Acidianus ambivalens DSM 3772 P29082
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.55
-
Acidianus ambivalens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.13.11.55 culture condition:aerobically-grown cell not detectable in anaerobically grown cells Acidianus ambivalens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.55 10.6
-
pH 7.4, 85°C Acidianus ambivalens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.55 4 sulfur + 4 H2O + O2 in the presence of oxygen but not under a hydrogen atmosphere, the enzyme simultaneously produces sulfite, thiosulfate, and hydrogen sulfide from sulfur. Nonenzymatic control experiments show that thiosulfate is produced mainly in a chemical reaction between sulfite and sulfur. The ratio of sulfite to hydrogen sulfide production is 5:4 in the presence of zinc ions Acidianus ambivalens 2 hydrogen sulfide + 2 bisulfite + 2 H+
-
?
1.13.11.55 4 sulfur + 4 H2O + O2 in the presence of oxygen but not under a hydrogen atmosphere, the enzyme simultaneously produces sulfite, thiosulfate, and hydrogen sulfide from sulfur. Nonenzymatic control experiments show that thiosulfate is produced mainly in a chemical reaction between sulfite and sulfur. The ratio of sulfite to hydrogen sulfide production is 5:4 in the presence of zinc ions Acidianus ambivalens DSM 3772 2 hydrogen sulfide + 2 bisulfite + 2 H+
-
?

Subunits

EC Number Subunits Comment Organism
1.13.11.55 ? x * 40000, SDS-PAGE Acidianus ambivalens

Synonyms

EC Number Synonyms Comment Organism
1.13.11.55 SOR
-
Acidianus ambivalens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.55 85
-
-
Acidianus ambivalens

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.13.11.55 75 100 75°C: about 40% of maximal activity Acidianus ambivalens