EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.13.11.2 | Ag+ | - |
Pseudomonas aeruginosa | |
1.13.11.2 | CuCl | - |
Pseudomonas aeruginosa | |
1.13.11.2 | CuSO4 | - |
Pseudomonas aeruginosa | |
1.13.11.2 | o-phenanthroline | - |
Pseudomonas aeruginosa |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.13.11.2 | 0.002 | - |
catechol | metapyrocatechase 2 | Pseudomonas aeruginosa | |
1.13.11.2 | 0.0041 | - |
catechol | metapyrocatechase 1 | Pseudomonas aeruginosa | |
1.13.11.2 | 0.008 | - |
4-methylcatechol | metapyrocatechase 2 | Pseudomonas aeruginosa | |
1.13.11.2 | 0.01 | - |
3-methylcatechol | metapyrocatechase 2 | Pseudomonas aeruginosa | |
1.13.11.2 | 0.02 | - |
4-methylcatechol | metapyrocatechase 1 | Pseudomonas aeruginosa | |
1.13.11.2 | 0.06 | - |
3-methylcatechol | metapyrocatechase 1 | Pseudomonas aeruginosa |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.13.11.2 | Fe2+ | FeCl2 and FeSO4 activate metapyrocatechase 2, no effect on metapyrocatechase 1 | Pseudomonas aeruginosa |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.13.11.2 | 33500 | - |
4 * 33500 metapyrocatechase 1, SDS-PAGE | Pseudomonas aeruginosa |
1.13.11.2 | 36000 | - |
4 * 36000, metapyrocatechase 2, SDS-PAGE | Pseudomonas aeruginosa |
1.13.11.2 | 132000 | 134000 | gel filtration, sedimentation equilibrium analysis, amino acid analysis, metapyrocatechase 2 | Pseudomonas aeruginosa |
1.13.11.2 | 143000 | 144600 | gel filtration, sedimentation equilibrium analysis, amino acid analysis | Pseudomonas aeruginosa |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.13.11.2 | catechol + O2 | Pseudomonas aeruginosa | metapyrocatechase 1 takes part in oxidation of aromatic compounds, producing catechol as an intermediate, metapyrocatechase 2 is involved in oxidation of methyl-substituted aromatic substrates | 2-hydroxymuconate semialdehyde | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.13.11.2 | Pseudomonas aeruginosa | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.13.11.2 | metapyrocatechase 1 and 2 | Pseudomonas aeruginosa |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.13.11.2 | 35 | - |
metapyrocatechase 2 | Pseudomonas aeruginosa |
1.13.11.2 | 90 | - |
metapyrocatechase 1 | Pseudomonas aeruginosa |
EC Number | Storage Stability | Organism |
---|---|---|
1.13.11.2 | -20°C, in lyophilized state for several months, metapyrocatechase 1 and 2 | Pseudomonas aeruginosa |
1.13.11.2 | 4°C, in acetone buffer under nitrogen for several weeks, metapyrocatechase 1 and 2 | Pseudomonas aeruginosa |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.13.11.2 | 3,4-dihydroxybenzoate + O2 | weak | Pseudomonas aeruginosa | ? | - |
? | |
1.13.11.2 | 3-chlorocatechol + O2 | weak | Pseudomonas aeruginosa | 2-chloro-2-hydroxy-6-oxohexa-2,4-dienoate | - |
? | |
1.13.11.2 | 3-methylcatechol + O2 | weak | Pseudomonas aeruginosa | 2-hydroxy-6-oxohepta-2,4-dienoate | - |
? | |
1.13.11.2 | 4-methylcatechol + O2 | - |
Pseudomonas aeruginosa | 2-hydroxy-3-methyl-6-oxohexa-2,4-dienoate | - |
? | |
1.13.11.2 | catechol + O2 | - |
Pseudomonas aeruginosa | 2-hydroxymuconate semialdehyde | - |
? | |
1.13.11.2 | catechol + O2 | metapyrocatechase 1 takes part in oxidation of aromatic compounds, producing catechol as an intermediate, metapyrocatechase 2 is involved in oxidation of methyl-substituted aromatic substrates | Pseudomonas aeruginosa | 2-hydroxymuconate semialdehyde | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.13.11.2 | tetramer | 4 * 33500 metapyrocatechase 1, SDS-PAGE | Pseudomonas aeruginosa |
1.13.11.2 | tetramer | 4 * 36000, metapyrocatechase 2, SDS-PAGE | Pseudomonas aeruginosa |