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Literature summary extracted from
- Properties of catachol-2,3-oxygenase from Pseudomonas aeruginosa (1976), Indian J. Biochem. Biophys., 13, 234-236.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 1.13.11.2 | molarity of buffer beyond 0.04 M not suitable | Pseudomonas aeruginosa |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.13.11.2 | acetone | - |
Pseudomonas aeruginosa |  |
| 1.13.11.2 | ATP | - |
Pseudomonas aeruginosa | |
| 1.13.11.2 | Mg2+ | - |
Pseudomonas aeruginosa | |
| 1.13.11.2 | Na2B4O7 | - |
Pseudomonas aeruginosa | |
| 1.13.11.2 | p-chloromercuribenzoate | - |
Pseudomonas aeruginosa | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.13.11.2 | catechol + O2 | Pseudomonas aeruginosa | metacleavage enzyme of catechol metabolism | 2-hydroxymuconate semialdehyde | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.13.11.2 | Pseudomonas aeruginosa | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.13.11.2 | partial | Pseudomonas aeruginosa |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.13.11.2 | 0-4°C, 0.02 M Tris-HCl, pH 8.0, fairly stable | Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.13.11.2 | catechol + O2 | - |
Pseudomonas aeruginosa | 2-hydroxymuconate semialdehyde | - |
? | |
| 1.13.11.2 | catechol + O2 | metacleavage enzyme of catechol metabolism | Pseudomonas aeruginosa | 2-hydroxymuconate semialdehyde | - |
? | |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.13.11.2 | 45 | - |
20 min, stable | Pseudomonas aeruginosa |
| 1.13.11.2 | 50 | - |
17 min, stable | Pseudomonas aeruginosa |
| 1.13.11.2 | 55 | - |
5 min, stable | Pseudomonas aeruginosa |
| 1.13.11.2 | 60 | - |
immediate loss of activity | Pseudomonas aeruginosa |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.13.11.2 | 7.5 | 8 | highest stability | Pseudomonas aeruginosa |
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