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Literature summary extracted from

  • Kumar, V.; Maresca, B.; Sacco, M.; Goewert, R.; Kobayashi, G.S.; Medoff, G.
    Purification and characterization of a cysteine dioxygenase from the yeast phase of Histoplasma capsulatum (1983), Biochemistry, 22, 762-768.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.13.11.20 NADH stimulates Histoplasma capsulatum

General Stability

EC Number General Stability Organism
1.13.11.20 pronase destroys activity Histoplasma capsulatum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.20 EDTA totally inhibits at very low concentrations Histoplasma capsulatum
1.13.11.20 EGTA totally inhibits at very low concentrations Histoplasma capsulatum
1.13.11.20 o-phenanthroline totally inhibits at very low concentrations Histoplasma capsulatum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.20 0.02
-
 L-cysteine
-
 Histoplasma capsulatum
1.13.11.20 16.6
-
 beta-mercaptoethanol
-
 Histoplasma capsulatum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.13.11.20 cytosol
-
 Histoplasma capsulatum 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.20 Fe stimulation, restores activity after inhibition with EDTA Histoplasma capsulatum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.20 10500
-
 gel filtration, SDS-PAGE, the estimate of molecular weight may be inaccurate because it is based on use of the iodinated protein and the assumption that iodination does not affect molecular weight Histoplasma capsulatum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.20 L-cysteine + O2 Histoplasma capsulatum probable role in the mycelial to yeast phase transition 3-sulfino-L-alanine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.20 Histoplasma capsulatum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.20 using filtration, centrifugation, column chromatography on DEAE-cellulose, Sephadex G-50 and cysteine-Sepharose Histoplasma capsulatum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.13.11.20 additional information no activity in mycelium Histoplasma capsulatum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.20 additional information
-
 6.31 atoms of oxygen/1000000 * min * mg protein Histoplasma capsulatum

Storage Stability

EC Number Storage Stability Organism
1.13.11.20 -70°C, crude enzyme, stable for up to 4 weeks Histoplasma capsulatum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.20 beta-mercaptoethanol + O2 slight activity Histoplasma capsulatum 2-hydroxyethanesulfinate
-
 ?
1.13.11.20 L-cysteine + O2 highly specific for L-cysteine Histoplasma capsulatum 3-sulfino-L-alanine
-
 ?
1.13.11.20 L-cysteine + O2 probable role in the mycelial to yeast phase transition Histoplasma capsulatum 3-sulfino-L-alanine
-
 ?
1.13.11.20 additional information D-cysteine, cystine, taurine, cystamine, cysteinesulfinic acid, glutathione, cysteic acid, S-methylcysteine and pyruvic acid do not serve as substrates Histoplasma capsulatum ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.20 monomer 1 * 10500, SDS-PAGE Histoplasma capsulatum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.20 37
-
 activity at 37°C 2-fold higher than at 25°C Histoplasma capsulatum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.13.11.20 25 37 activity at 37°C 2-fold higher than at 25°C Histoplasma capsulatum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.20 8
-
-
 Histoplasma capsulatum

Cofactor

EC Number Cofactor Comment Organism Structure
1.13.11.20 FAD 1 mol of enzyme contains about 0.1 mol of flavin Histoplasma capsulatum