BRENDA - Enzyme Database show

2,3-Dihydroxybenzoate 2,3-oxygease from the chloroplast fraction of Tecoma stans

Sharma, H.K.; Vaidyanathan, C.S.; Phytochemistry 14, 2135-2139 (1975)
No PubMed abstract available

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
1.13.11.28
chloroplast enzyme more stable than soluble enzyme
Tecoma stans
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.13.11.28
8-hydroxyquinoline
reversible by addition of Cu2+
Tecoma stans
1.13.11.28
Ag+
-
Tecoma stans
1.13.11.28
arsenite
-
Tecoma stans
1.13.11.28
azide
-
Tecoma stans
1.13.11.28
Cd2+
-
Tecoma stans
1.13.11.28
CN-
-
Tecoma stans
1.13.11.28
Co2+
-
Tecoma stans
1.13.11.28
diethyldithiocarbamate
reversible by addition of Cu2+
Tecoma stans
1.13.11.28
F-
-
Tecoma stans
1.13.11.28
Fe2+
-
Tecoma stans
1.13.11.28
Fe3+
-
Tecoma stans
1.13.11.28
glutathione
no inhibition of chloroplast enzyme
Tecoma stans
1.13.11.28
Hg2+
-
Tecoma stans
1.13.11.28
Mg2+
no inhibition of chloroplast enzyme
Tecoma stans
1.13.11.28
N-ethylmaleimide
-
Tecoma stans
1.13.11.28
Neocuproine
reversible by addition of Cu2+
Tecoma stans
1.13.11.28
p-chloromercuribenzoate
-
Tecoma stans
1.13.11.28
Salicylaldoxime
reversible by addition of Cu2+
Tecoma stans
1.13.11.28
Semicarbazide
-
Tecoma stans
1.13.11.28
Sulfide
-
Tecoma stans
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.13.11.28
chloroplast
-
Tecoma stans
9507
-
1.13.11.28
soluble
-
Tecoma stans
-
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.13.11.28
2,3-dihydroxybenzoate + O2
Tecoma stans
enzyme of the beta-ketoadipate pathway
2-carboxy-cis,cis-muconate
-
Tecoma stans
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.13.11.28
Tecoma stans
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.13.11.28
leaf
-
Tecoma stans
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.13.11.28
2,3-dihydroxybenzoate + O2
-
439516
Tecoma stans
2-carboxy-cis,cis-muconate
-
439516
Tecoma stans
?
1.13.11.28
2,3-dihydroxybenzoate + O2
enzyme of the beta-ketoadipate pathway
439516
Tecoma stans
2-carboxy-cis,cis-muconate
-
439516
Tecoma stans
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.13.11.28
30
-
-
Tecoma stans
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
1.13.11.28
60
-
30% of activity
Tecoma stans
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.13.11.28
-
-
half-life of chloroplast enzyme is 8-9 h
Tecoma stans
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.13.11.28
5.6
-
-
Tecoma stans
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.13.11.28
7
-
no activity above pH 7
Tecoma stans
General Stability (protein specific)
EC Number
General Stability
Organism
1.13.11.28
chloroplast enzyme more stable than soluble enzyme
Tecoma stans
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.13.11.28
8-hydroxyquinoline
reversible by addition of Cu2+
Tecoma stans
1.13.11.28
Ag+
-
Tecoma stans
1.13.11.28
arsenite
-
Tecoma stans
1.13.11.28
azide
-
Tecoma stans
1.13.11.28
Cd2+
-
Tecoma stans
1.13.11.28
CN-
-
Tecoma stans
1.13.11.28
Co2+
-
Tecoma stans
1.13.11.28
diethyldithiocarbamate
reversible by addition of Cu2+
Tecoma stans
1.13.11.28
F-
-
Tecoma stans
1.13.11.28
Fe2+
-
Tecoma stans
1.13.11.28
Fe3+
-
Tecoma stans
1.13.11.28
glutathione
no inhibition of chloroplast enzyme
Tecoma stans
1.13.11.28
Hg2+
-
Tecoma stans
1.13.11.28
Mg2+
no inhibition of chloroplast enzyme
Tecoma stans
1.13.11.28
N-ethylmaleimide
-
Tecoma stans
1.13.11.28
Neocuproine
reversible by addition of Cu2+
Tecoma stans
1.13.11.28
p-chloromercuribenzoate
-
Tecoma stans
1.13.11.28
Salicylaldoxime
reversible by addition of Cu2+
Tecoma stans
1.13.11.28
Semicarbazide
-
Tecoma stans
1.13.11.28
Sulfide
-
Tecoma stans
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.13.11.28
chloroplast
-
Tecoma stans
9507
-
1.13.11.28
soluble
-
Tecoma stans
-
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.13.11.28
2,3-dihydroxybenzoate + O2
Tecoma stans
enzyme of the beta-ketoadipate pathway
2-carboxy-cis,cis-muconate
-
Tecoma stans
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.13.11.28
leaf
-
Tecoma stans
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.13.11.28
2,3-dihydroxybenzoate + O2
-
439516
Tecoma stans
2-carboxy-cis,cis-muconate
-
439516
Tecoma stans
?
1.13.11.28
2,3-dihydroxybenzoate + O2
enzyme of the beta-ketoadipate pathway
439516
Tecoma stans
2-carboxy-cis,cis-muconate
-
439516
Tecoma stans
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.13.11.28
30
-
-
Tecoma stans
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
1.13.11.28
60
-
30% of activity
Tecoma stans
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.13.11.28
-
-
half-life of chloroplast enzyme is 8-9 h
Tecoma stans
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.13.11.28
5.6
-
-
Tecoma stans
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.13.11.28
7
-
no activity above pH 7
Tecoma stans