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Literature summary extracted from

  • Sharma, H.K.; Vaidyanathan, C.S.
    A new mode of ring cleavage of 2,3-dihydroxybenzoic acid in Tecoma stans (L.). Partial purification and properties of 2,3-dihydroxybenzoate 2,3-oxygenase (1975), Eur. J. Biochem., 56, 163-171.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.28 2-mercaptoethanol preincubation with substrate protects against inactivation Tecoma stans
1.13.11.28 8-hydroxyquinoline reversible by addition of Cu2+ Tecoma stans
1.13.11.28 Ag+
-
 Tecoma stans
1.13.11.28 azide
-
 Tecoma stans
1.13.11.28 CN-
-
 Tecoma stans
1.13.11.28 diethyldithiocarbamate reversible by addition of Cu2+ Tecoma stans
1.13.11.28 dithiothreitol preincubation with substrate protects against inactivation Tecoma stans
1.13.11.28 Fe2+
-
 Tecoma stans
1.13.11.28 Fe3+
-
 Tecoma stans
1.13.11.28 glutathione reduced form, preincubation with substrate protects against inactivation Tecoma stans
1.13.11.28 Hg2+
-
 Tecoma stans
1.13.11.28 iodoacetate preincubation with substrate protects against inactivation Tecoma stans
1.13.11.28 L-cysteine preincubation with substrate protects against inactivation Tecoma stans
1.13.11.28 Mg2+
-
 Tecoma stans
1.13.11.28 N-ethylmaleimide preincubation with substrate protects against inactivation Tecoma stans
1.13.11.28 Neocuproine reversible by addition of Cu2+ Tecoma stans
1.13.11.28 p-chloromercuribenzoate preincubation with substrate protects against inactivation Tecoma stans
1.13.11.28 Salicylaldoxime reversible by addition of Cu2+ Tecoma stans
1.13.11.28 Sulfide
-
 Tecoma stans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.28 65
-
 2,3-Dihydroxybenzoate
-
 Tecoma stans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.28 Cu2+
-
 Tecoma stans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.28 2,3-dihydroxybenzoate + O2 Tecoma stans
-
 2-carboxy-cis,cis-muconate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.28 Tecoma stans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.28 partial Tecoma stans

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.13.11.28 leaf
-
 Tecoma stans
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.28 2,3-dihydroxy-p-cumate + O2 28% of activity Tecoma stans 2-carboxy-4-isopropyl-cis,cis-muconate
-
 ?
1.13.11.28 2,3-dihydroxy-p-toluate + O2 38% of activity Tecoma stans 2-carboxy-4-methyl-cis,cis-muconate
-
 ?
1.13.11.28 2,3-dihydroxybenzoate + O2
-
 Tecoma stans 2-carboxy-cis,cis-muconate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.28 30
-
-
 Tecoma stans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.13.11.28 -20
-
 cannot be stored at -20°C except as ammonium sulfate precipitate or adsorbed to tricalcium phosphate gel Tecoma stans
1.13.11.28
-
-
 extremely labile, crude preparation loses 50% of activity within 3-4 h at pH 5.2 Tecoma stans
1.13.11.28 55
-
 complete inactivation after 20 min, addition of 0.006 mM substrate protects against inactivation for 15 min Tecoma stans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.28 5.2
-
-
 Tecoma stans