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Literature summary extracted from
- Uridine diphosphate D-glucuronic acid cyclase and uridine diphosphate D-glucuronic acid carboxy-lyase I from Lemna minor. Purification, characterization, and separation from uridine diphosphate D-glucuronic acid carboxy-lyase II (1975), Arch. Biochem. Biophys., 170, 82-91.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.1.1.35 | NH4Cl | activates | Lemna minor |  |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 4.1.1.35 | stable when stored at -90°C and thawed and frozen three times, at days 0, 50 and 100 | Lemna minor |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.1.1.35 | iodoacetamide | - |
Lemna minor | |
| 4.1.1.35 | KCl | - |
Lemna minor | |
| 4.1.1.35 | NEM | - |
Lemna minor | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.1.1.35 | 0.0083 | - |
UDPglucuronate | - |
Lemna minor | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.1.1.35 | Lemna minor | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.1.1.35 | - |
Lemna minor |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.1.1.35 | 4.98 | - |
- |
Lemna minor |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 4.1.1.35 | -20°C or -90°C, 60% w/v glycerol, stable for at least 100 days | Lemna minor |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.1.1.35 | UDP-glucuronate | - |
Lemna minor | UDP-xylose + CO2 | - |
? | |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.1.1.35 | 20 | - |
rapid inactivation without glycerol | Lemna minor |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.1.1.35 | 7.7 | - |
- |
Lemna minor |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.1.1.35 | NAD+ | required | Lemna minor | |
| 4.1.1.35 | NAD+ | optimal concentration: 1-2 mM | Lemna minor | |
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