Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Mohan, V.P.; Kishore, G.; Sugumaran, M.; Vaidyanathan, C.S.
    Purification and properties of protocatechuate-3,4-dioxygenase from Tecoma stans L. (1979), Plant Sci. Lett., 16, 267-272.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.3 4-hydroxymercuribenzoate 0.1 mM, complete inhibition Tecoma stans
1.13.11.3 iodoacetate 0.1 mM, complete inhibition Tecoma stans
1.13.11.3 N-ethylmaleimide 0.1 mM, complete inhibition Tecoma stans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.3 0.04
-
FeSO4
-
Tecoma stans
1.13.11.3 0.33
-
protocatechuate
-
Tecoma stans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.13.11.3 cytosol localized in 100000 g supernatant, completely absent in mitochondrial, microsomal and chloroplast fraction Tecoma stans 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.3 Fe external addition of FeSO4 is absolutely essential, other metal ions cannot replace Fe2+ Tecoma stans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.3 150000
-
gel filtration Tecoma stans

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.3 Tecoma stans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.3 ammonium sulfate, DEAE-cellulose Tecoma stans

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.13.11.3 leaf
-
Tecoma stans
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.3 0.0081
-
-
Tecoma stans

Storage Stability

EC Number Storage Stability Organism
1.13.11.3 stable only 7 days Tecoma stans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.3 3,4-dihydroxybenzoate + O2 no other substrate found Tecoma stans 3-carboxy-cis,cis-muconate
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.3 40
-
-
Tecoma stans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.13.11.3 60
-
5 min, stable Tecoma stans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.3 5.2
-
completely inactive above pH 6.5 Tecoma stans