Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Furukawa, M.; Yoshimoto, T.; Ochi, K.; Yamamoto, S.
    Studies on arachidonate 5-lipoxygenase of rat basophilic leukemia cells (1984), Biochim. Biophys. Acta, 795, 458-465.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.13.11.34 ADP about half as active as ATP Rattus norvegicus
1.13.11.34 AMP about half as active as ATP Rattus norvegicus
1.13.11.34 ATP stimulates Rattus norvegicus
1.13.11.34 CTP about half as active as ATP Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.34 1,10-phenanthroline 0.01 mM, 34% inhibition Rattus norvegicus
1.13.11.34 5,8,11,13-eicosatetraynoic acid IC50: 0.028 mM Rattus norvegicus
1.13.11.34 8-hydroxyquinoline 0.01 mM, 59% inhibition Rattus norvegicus
1.13.11.34 AA861 IC50: 0.0026 mM Rattus norvegicus
1.13.11.34 baicalein IC50: 0.0012 mM Rattus norvegicus
1.13.11.34 BW755C CAS: 66000-40-6; IC50: 0.53 MM Rattus norvegicus
1.13.11.34 caffeic acid IC50: 0.046 mM Rattus norvegicus
1.13.11.34 catechol IC50: 0.062 mM Rattus norvegicus
1.13.11.34 Cirsiliol IC50: 0.098 mM Rattus norvegicus
1.13.11.34 epinephrine IC50: 0.98 mM Rattus norvegicus
1.13.11.34 esculetin IC50: 0.0045 mM Rattus norvegicus
1.13.11.34 eupatilin IC50: 0.098 mM Rattus norvegicus
1.13.11.34 indomethacin IC50: 0.36 mM Rattus norvegicus
1.13.11.34 iodoacetamide 0.01 mM, 20% inhibition Rattus norvegicus
1.13.11.34 NEM 0.01 mM, 75% inhibition Rattus norvegicus
1.13.11.34 nordihydroguiaraetic acid IC50: 0.00021 mM Rattus norvegicus
1.13.11.34 norepinephrine IC50: 0.49 mM Rattus norvegicus
1.13.11.34 quercetin IC50: 0.0021 mM Rattus norvegicus
1.13.11.34 rutin IC50: 0.033 mM Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.34 0.017
-
 arachidonic acid
-
 Rattus norvegicus
1.13.11.34 0.024
-
 5,8,11,14,17-eicosapentaenoic acid
-
 Rattus norvegicus
1.13.11.34 0.032
-
 5,8,11-eicosatrienoic acid
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.13.11.34 cytosol
-
 Rattus norvegicus 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.34 Ba2+ 45% of the activation with Ca2+ Rattus norvegicus
1.13.11.34 Ca2+ required Rattus norvegicus
1.13.11.34 Ca2+ half-maximal activity at 0.1 mM Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.34 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.34 partial Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.13.11.34 leukemia cell
-
 Rattus norvegicus
-

Storage Stability

EC Number Storage Stability Organism
1.13.11.34 4°C, 48 h, no appreciable loss of activity Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.34 5,8,11,14,17-eicosapentaenoic acid + O2
-
 Rattus norvegicus ?
-
 ?
1.13.11.34 5,8,11-eicosatrienoic acid + O2 almost as active as arachidonic acid Rattus norvegicus ?
-
 ?
1.13.11.34 8,11,14-eicosatrienoic acid + O2
-
 Rattus norvegicus ?
-
 ?
1.13.11.34 arachidonate + O2 i.e. 5,8,11,14-eicosatetraenoic acid Rattus norvegicus (6E,8Z,11Z,14Z)-(5S)-hydroperoxyicosa-6,8,11,14-tetraenoate
-
 ?

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.13.11.34 0.00021
-
 IC50: 0.00021 mM Rattus norvegicus nordihydroguiaraetic acid
1.13.11.34 0.0012
-
 IC50: 0.0012 mM Rattus norvegicus baicalein
1.13.11.34 0.0021
-
 IC50: 0.0021 mM Rattus norvegicus quercetin
1.13.11.34 0.0026
-
 IC50: 0.0026 mM Rattus norvegicus AA861
1.13.11.34 0.0045
-
 IC50: 0.0045 mM Rattus norvegicus esculetin
1.13.11.34 0.028
-
 IC50: 0.028 mM Rattus norvegicus 5,8,11,13-eicosatetraynoic acid
1.13.11.34 0.033
-
 IC50: 0.033 mM Rattus norvegicus rutin
1.13.11.34 0.046
-
 IC50: 0.046 mM Rattus norvegicus caffeic acid
1.13.11.34 0.062
-
 IC50: 0.062 mM Rattus norvegicus catechol
1.13.11.34 0.098
-
 IC50: 0.098 mM Rattus norvegicus Cirsiliol
1.13.11.34 0.098
-
 IC50: 0.098 mM Rattus norvegicus eupatilin
1.13.11.34 0.36
-
 IC50: 0.36 mM Rattus norvegicus indomethacin
1.13.11.34 0.49
-
 IC50: 0.49 mM Rattus norvegicus norepinephrine
1.13.11.34 0.53
-
 IC50: 0.53 mM Rattus norvegicus BW755C
1.13.11.34 0.98
-
 IC50: 0.98 mM Rattus norvegicus epinephrine