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Literature summary extracted from

  • Reddanna, P.; Whelan, J.; Maddipati, K.R.; Reddy, C.C.
    Purification of arachidonate 5-lipoxygenase from potato tubers (1990), Methods Enzymol., 187, 268-277.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.34 0.03
-
 arachidonic acid
-
 Solanum tuberosum
1.13.11.34 0.033
-
 arachidonic acid
-
 Solanum tuberosum
1.13.11.34 0.05
-
 bishomo-gamma-linolenic acid
-
 Solanum tuberosum
1.13.11.34 0.2
-
 linoleic acid
-
 Solanum tuberosum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.34 35000
-
 x * 35000, enzyme form L2, SDS-PAGE Solanum tuberosum
1.13.11.34 85000
-
 enzyme form L1 and L2, gel filtration Solanum tuberosum

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.34 Solanum tuberosum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.34
-
 Solanum tuberosum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.13.11.34 tuber
-
 Solanum tuberosum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.34 5.7
-
 enzyme form L2 Solanum tuberosum
1.13.11.34 13.7
-
 enzyme form L1 Solanum tuberosum

Storage Stability

EC Number Storage Stability Organism
1.13.11.34 -20°C, 40 mM potassium phosphate buffer, pH 6.3, 2 M (NH4)2SO4, enzyme form L1 is stable for months Solanum tuberosum
1.13.11.34 4°C, the L1 form is gradually transformed into the L2 form with a significant reduction in specific activity, the L2 form loses all of its activity over a few days and more rapid in absence of (NH4)2SO4 Solanum tuberosum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.34 arachidonate + O2 i.e. 5,8,11,14-eicosatetraenoic acid Solanum tuberosum (6E,8Z,11Z,14Z)-(5S)-hydroperoxyicosa-6,8,11,14-tetraenoate 5(S)-hydroperoxyeicosatetraenoic acid is the most abundant primary oxygenation product, representing 60% of the total hydroperoxyeicosatetraenoic acids, followed by 8(S)-hydroperoxyeicosatetraenoic acid, 9-hydroperoxyeicosatetraenoic acid, 11(S)-hydroperoxyeicosatetraenoic acid, 12-hydroperoxyeicosatetraenoic acid, and 15-hydroperoxyeicosatetraenoic acid in decreasing order of abundance, all possible diH(P)ETEs are detected ?
1.13.11.34 bishomo-gamma-linolenic acid + O2
-
 Solanum tuberosum ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.34 ? x * 35000, enzyme form L2, SDS-PAGE Solanum tuberosum
1.13.11.34 ? x * 85000, enzyme form L2, SDS-PAGE Solanum tuberosum
1.13.11.34 More the L1 form is gradually transformed into the L2 form with a significant reduction in specific activity Solanum tuberosum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.34 additional information
-
 pH of the reaction has profound influence on the product profile Solanum tuberosum