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Literature summary extracted from

  • Denis, D.; Falgueyret, J.P.; Riendeau, D.; Abramovitz, M.
    Characterization of the activity of purified recombinant human 5-lipoxygenase in the absence and presence of leukocyte factors (1991), J. Biol. Chem., 266, 5072-5079.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.13.11.34 protein factors from human leukocyte stimulates Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.34 high expression baculovirus system Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.34 BW755C CAS: 66000-40-6; i.e. 3-amino-1-[3-(trifluoromethyl)phenyl]-2-pyrazoline, IC50: 0.013 mM Homo sapiens
1.13.11.34 L-656,224 i.e. 7-chloro-2-[(4-methoxyphenyl)-methyl]-3-methyl-5-propyl-4-benzofuranol, IC50: 0.0008 mM Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.34 Ca2+ above 0.1 mM, required for maximal activity Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.34 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.34 recombinant enzyme Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.13.11.34 leukocyte
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.34 0.006
-
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.34 arachidonate + O2 i.e. 5,8,11,14-eicosatetraenoic acid Homo sapiens (6E,8Z,11Z,14Z)-(5S)-hydroperoxyicosa-6,8,11,14-tetraenoate 5(S)-hydroperoxy-6-trans-8,11,14-cis-eicosatetraenoic acid + 6-trans-leukotriene B4 + 12-epi-6-trans-leukotriene B4 ?
1.13.11.34 additional information the purified protein expresses both 5-oxygenase and leukotriene A4 synthase activities in the ratio of 6:1 Homo sapiens ?
-
 ?

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.13.11.34 0.0008
-
 i.e. 7-chloro-2-[(4-methoxyphenyl)-methyl]-3-methyl-5-propyl-4-benzofuranol, IC50: 0.0008 mM Homo sapiens L-656,224
1.13.11.34 0.013
-
 i.e. 3-amino-1-[3-(trifluoromethyl)phenyl]-2-pyrazoline, IC50: 0.013 mM Homo sapiens BW755C