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Literature summary extracted from

  • Zaborina, O.; Latus, M.; Eberspaecher, J.; Golovleva, L.A.; Lingens, F.
    Purification and characterization of 6-chlorohydroxyquinol 1,2-dioxygenase from Streptomyces rochei 303: comparison with an analogous enzyme from Azotobacter sp. strain GP1 (1995), J. Bacteriol., 177, 229-234.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.37 0.02
-
 Hydroxyquinol
-
 Streptomyces rochei
1.13.11.37 0.06
-
 6-chlorohydroxyquinol
-
 Streptomyces rochei

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.37 Iron 1 mol of iron per mol enzyme Streptomyces rochei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.37 31000
-
 2 * 31000, SDS-PAGE Streptomyces rochei
1.13.11.37 61000
-
 gel filtration Streptomyces rochei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.37 benzene-1,2,4-triol + O2 Streptomyces rochei
-
 3-hydroxy-cis-cis-muconate
-
 ?
1.13.11.37 benzene-1,2,4-triol + O2 Streptomyces rochei DSM 40242
-
 3-hydroxy-cis-cis-muconate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.37 Streptomyces rochei
-
 strain 303 VKM AC-1284D, grown with 2,4,6-trichlorophenol
-
1.13.11.37 Streptomyces rochei DSM 40242
-
 strain 303 VKM AC-1284D, grown with 2,4,6-trichlorophenol
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.37
-
 Streptomyces rochei

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.37 27
-
-
 Streptomyces rochei

Storage Stability

EC Number Storage Stability Organism
1.13.11.37 4°C, reasonably stable for several weeks in the presence of isopropanol Streptomyces rochei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.37 6-chlorohydroxyquinol + O2
-
 Streptomyces rochei 2-chloromaleylacetate
-
 ?
1.13.11.37 6-chlorohydroxyquinol + O2
-
 Streptomyces rochei DSM 40242 2-chloromaleylacetate
-
 ?
1.13.11.37 benzene-1,2,4-triol + O2
-
 Streptomyces rochei maleylacetate
-
 ?
1.13.11.37 benzene-1,2,4-triol + O2
-
 Streptomyces rochei DSM 40242 maleylacetate
-
 ?
1.13.11.37 benzene-1,2,4-triol + O2
-
 Streptomyces rochei 3-hydroxy-cis-cis-muconate
-
 ?
1.13.11.37 benzene-1,2,4-triol + O2
-
 Streptomyces rochei DSM 40242 3-hydroxy-cis-cis-muconate
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.37 homodimer 2 * 31000, SDS-PAGE Streptomyces rochei

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.11.37 11.4
-
 Hydroxyquinol
-
 Streptomyces rochei
1.13.11.37 72.3
-
 6-chlorohydroxyquinol
-
 Streptomyces rochei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.37 6.6
-
-
 Streptomyces rochei

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.13.11.37 6.2 7.6
-
 Streptomyces rochei