EC Number | General Stability | Organism |
---|---|---|
1.14.13.1 | dithiothreitol, 0.5 mM, stabilizes | Cutaneotrichosporon cutaneum |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.1 | Cl- | - |
Cutaneotrichosporon cutaneum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.13.11.37 | 0.0057 | - |
Hydroxyquinol | - |
Cutaneotrichosporon cutaneum | |
1.14.13.1 | additional information | - |
additional information | - |
Cutaneotrichosporon cutaneum |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.13.11.37 | Iron | - |
Cutaneotrichosporon cutaneum | |
1.14.13.1 | additional information | no metal ion requirement | Cutaneotrichosporon cutaneum |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.13.11.37 | 38200 | - |
alpha 1 * 39600 + beta 1 * 38200, SDS-PAGE | Cutaneotrichosporon cutaneum |
1.13.11.37 | 39600 | - |
alpha 1 * 39600 + beta 1 * 38200, SDS-PAGE | Cutaneotrichosporon cutaneum |
1.13.11.37 | 93000 | - |
gel filtration | Cutaneotrichosporon cutaneum |
1.14.13.1 | 43400 | 45300 | gel filtration, SDS-PAGE | Cutaneotrichosporon cutaneum |
1.14.13.1 | 45300 | - |
1 * 45300 SDS-PAGE | Cutaneotrichosporon cutaneum |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.13.11.37 | benzene-1,2,4-triol + O2 | Cutaneotrichosporon cutaneum | - |
3-hydroxy-cis-cis-muconate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.13.11.37 | Cutaneotrichosporon cutaneum | - |
grown with hydrobenzoate | - |
1.14.13.1 | Cutaneotrichosporon cutaneum | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.13.1 | - |
Cutaneotrichosporon cutaneum |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.13.11.37 | 140 | - |
- |
Cutaneotrichosporon cutaneum |
1.14.13.1 | 37 | - |
- |
Cutaneotrichosporon cutaneum |
EC Number | Storage Stability | Organism |
---|---|---|
1.13.11.37 | 4°C, loss of most of the activity after 1 week | Cutaneotrichosporon cutaneum |
1.14.13.1 | 4°C, 0.5 mM dithiothreitol, 10% loss of activity after 1 week | Cutaneotrichosporon cutaneum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.13.11.37 | benzene-1,2,4-triol + O2 | - |
Cutaneotrichosporon cutaneum | maleylacetate | - |
? | |
1.13.11.37 | benzene-1,2,4-triol + O2 | - |
Cutaneotrichosporon cutaneum | 3-hydroxy-cis-cis-muconate | - |
? | |
1.14.13.1 | 2,3-dihydroxybenzoate + NADH + O2 | - |
Cutaneotrichosporon cutaneum | pyrogallol + CO2 + H2O + NAD+ | - |
? | |
1.14.13.1 | 2,4-dihydroxybenzoate + NAD(P)H + O2 | - |
Cutaneotrichosporon cutaneum | benzene-1,2,4-triol + CO2 + H2O + NAD(P)+ | - |
? | |
1.14.13.1 | 2,5-dihydroxybenzoate + NADH + O2 | or NADPH | Cutaneotrichosporon cutaneum | benzene-1,2,5-triol + CO2 + H2O + NAD+ | - |
? | |
1.14.13.1 | 2,6-dihydroxybenzoate + NADH + O2 | - |
Cutaneotrichosporon cutaneum | pyrogallol + CO2 + H2O + NAD+ | - |
? | |
1.14.13.1 | 4-aminosalicylate + NADH + O2 | - |
Cutaneotrichosporon cutaneum | 4-aminocatechol + CO2 + NAD+ + H2O | - |
? | |
1.14.13.1 | 4-chlorosalicylate + NADH + O2 | - |
Cutaneotrichosporon cutaneum | 1,2-dihydroxy-4-chlorobenzene + CO2 + NAD+ + H2O | - |
? | |
1.14.13.1 | 5-aminosalicylate + NADH + O2 | - |
Cutaneotrichosporon cutaneum | 5-aminocatechol + CO2 + NAD+ + H2O | - |
? | |
1.14.13.1 | 5-chlorosalicylate + NADH + O2 | - |
Cutaneotrichosporon cutaneum | 1,2-dihydroxy-5-chlorobenzene + CO2 + NAD+ + H2O | - |
? | |
1.14.13.1 | 5-fluorosalicylate + NADH + O2 | - |
Cutaneotrichosporon cutaneum | 1,2-dihydroxy-5-fluorobenzene + CO2 + NAD+ + H2O | - |
? | |
1.14.13.1 | 5-methoxysalicylate + NADH + O2 | - |
Cutaneotrichosporon cutaneum | 1,2-dihydroxy-5-methoxybenzene + CO2 + NAD+ + H2O | - |
? | |
1.14.13.1 | 5-methylsalicylate + NADH + H+ + O2 | - |
Cutaneotrichosporon cutaneum | 1,2-dihydroxy-5-methylbenzene + CO2 + NAD+ + H2O | - |
? | |
1.14.13.1 | salicylate + NADH + H+ + O2 | - |
Cutaneotrichosporon cutaneum | catechol + NAD+ + H2O + CO2 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.13.11.37 | heterodimer | alpha 1 * 39600 + beta 1 * 38200, SDS-PAGE | Cutaneotrichosporon cutaneum |
1.14.13.1 | monomer | 1 * 45300 SDS-PAGE | Cutaneotrichosporon cutaneum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.13.11.37 | 6 | - |
enzyme assay | Cutaneotrichosporon cutaneum |
1.14.13.1 | 7.5 | - |
- |
Cutaneotrichosporon cutaneum |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.14.13.1 | 6.5 | 8.5 | about 75% of activity maximum at pH 6.5 and 8.5 | Cutaneotrichosporon cutaneum |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.1 | FAD | flavoprotein | Cutaneotrichosporon cutaneum | |
1.14.13.1 | NADH | - |
Cutaneotrichosporon cutaneum | |
1.14.13.1 | NADPH | 60% of the activity with NADH | Cutaneotrichosporon cutaneum |