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Literature summary extracted from

  • Sze, I.S.Y.; Dagley, S.
    Properties of salicylate hydroxylase and hydroxyquinol 1,2-dioxygenase purified from Trichosporon cutaneum (1984), J. Bacteriol., 159, 353-359.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
1.14.13.1 dithiothreitol, 0.5 mM, stabilizes Cutaneotrichosporon cutaneum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.13.1 Cl-
-
Cutaneotrichosporon cutaneum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.37 0.0057
-
Hydroxyquinol
-
Cutaneotrichosporon cutaneum
1.14.13.1 additional information
-
additional information
-
Cutaneotrichosporon cutaneum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.37 Iron
-
Cutaneotrichosporon cutaneum
1.14.13.1 additional information no metal ion requirement Cutaneotrichosporon cutaneum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.37 38200
-
alpha 1 * 39600 + beta 1 * 38200, SDS-PAGE Cutaneotrichosporon cutaneum
1.13.11.37 39600
-
alpha 1 * 39600 + beta 1 * 38200, SDS-PAGE Cutaneotrichosporon cutaneum
1.13.11.37 93000
-
gel filtration Cutaneotrichosporon cutaneum
1.14.13.1 43400 45300 gel filtration, SDS-PAGE Cutaneotrichosporon cutaneum
1.14.13.1 45300
-
1 * 45300 SDS-PAGE Cutaneotrichosporon cutaneum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.37 benzene-1,2,4-triol + O2 Cutaneotrichosporon cutaneum
-
3-hydroxy-cis-cis-muconate
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.37 Cutaneotrichosporon cutaneum
-
grown with hydrobenzoate
-
1.14.13.1 Cutaneotrichosporon cutaneum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.1
-
Cutaneotrichosporon cutaneum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.37 140
-
-
Cutaneotrichosporon cutaneum
1.14.13.1 37
-
-
Cutaneotrichosporon cutaneum

Storage Stability

EC Number Storage Stability Organism
1.13.11.37 4°C, loss of most of the activity after 1 week Cutaneotrichosporon cutaneum
1.14.13.1 4°C, 0.5 mM dithiothreitol, 10% loss of activity after 1 week Cutaneotrichosporon cutaneum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.37 benzene-1,2,4-triol + O2
-
Cutaneotrichosporon cutaneum maleylacetate
-
?
1.13.11.37 benzene-1,2,4-triol + O2
-
Cutaneotrichosporon cutaneum 3-hydroxy-cis-cis-muconate
-
?
1.14.13.1 2,3-dihydroxybenzoate + NADH + O2
-
Cutaneotrichosporon cutaneum pyrogallol + CO2 + H2O + NAD+
-
?
1.14.13.1 2,4-dihydroxybenzoate + NAD(P)H + O2
-
Cutaneotrichosporon cutaneum benzene-1,2,4-triol + CO2 + H2O + NAD(P)+
-
?
1.14.13.1 2,5-dihydroxybenzoate + NADH + O2 or NADPH Cutaneotrichosporon cutaneum benzene-1,2,5-triol + CO2 + H2O + NAD+
-
?
1.14.13.1 2,6-dihydroxybenzoate + NADH + O2
-
Cutaneotrichosporon cutaneum pyrogallol + CO2 + H2O + NAD+
-
?
1.14.13.1 4-aminosalicylate + NADH + O2
-
Cutaneotrichosporon cutaneum 4-aminocatechol + CO2 + NAD+ + H2O
-
?
1.14.13.1 4-chlorosalicylate + NADH + O2
-
Cutaneotrichosporon cutaneum 1,2-dihydroxy-4-chlorobenzene + CO2 + NAD+ + H2O
-
?
1.14.13.1 5-aminosalicylate + NADH + O2
-
Cutaneotrichosporon cutaneum 5-aminocatechol + CO2 + NAD+ + H2O
-
?
1.14.13.1 5-chlorosalicylate + NADH + O2
-
Cutaneotrichosporon cutaneum 1,2-dihydroxy-5-chlorobenzene + CO2 + NAD+ + H2O
-
?
1.14.13.1 5-fluorosalicylate + NADH + O2
-
Cutaneotrichosporon cutaneum 1,2-dihydroxy-5-fluorobenzene + CO2 + NAD+ + H2O
-
?
1.14.13.1 5-methoxysalicylate + NADH + O2
-
Cutaneotrichosporon cutaneum 1,2-dihydroxy-5-methoxybenzene + CO2 + NAD+ + H2O
-
?
1.14.13.1 5-methylsalicylate + NADH + H+ + O2
-
Cutaneotrichosporon cutaneum 1,2-dihydroxy-5-methylbenzene + CO2 + NAD+ + H2O
-
?
1.14.13.1 salicylate + NADH + H+ + O2
-
Cutaneotrichosporon cutaneum catechol + NAD+ + H2O + CO2
-
?

Subunits

EC Number Subunits Comment Organism
1.13.11.37 heterodimer alpha 1 * 39600 + beta 1 * 38200, SDS-PAGE Cutaneotrichosporon cutaneum
1.14.13.1 monomer 1 * 45300 SDS-PAGE Cutaneotrichosporon cutaneum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.37 6
-
enzyme assay Cutaneotrichosporon cutaneum
1.14.13.1 7.5
-
-
Cutaneotrichosporon cutaneum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.14.13.1 6.5 8.5 about 75% of activity maximum at pH 6.5 and 8.5 Cutaneotrichosporon cutaneum

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.1 FAD flavoprotein Cutaneotrichosporon cutaneum
1.14.13.1 NADH
-
Cutaneotrichosporon cutaneum
1.14.13.1 NADPH 60% of the activity with NADH Cutaneotrichosporon cutaneum