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Literature summary extracted from

  • Brash, A.R.; Boeglin, W.E.; Chang, M.S.; Shieh, B.H.
    Purification and molecular cloning of an 8R-lipoxygenase from the coral Plexaura homomalla reveal the related primary structures of R- and S-lipoxygenases (1996), J. Biol. Chem., 271, 20949-20957.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.40 full-length cDNA cloned by PCR Plexaura homomalla

General Stability

EC Number General Stability Organism
1.13.11.40 unstable in solution, activity is completely lost after standing on ice overnight Plexaura homomalla

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.40 76000
-
 mature protein predicted from the cDNA, difference from the size estimated by SDS-PAGE implies a post-translational modification of the enzyme Plexaura homomalla
1.13.11.40 100000
-
 SDS-PAGE Plexaura homomalla

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.40 2 arachidonic acid + 2 O2 Strongylocentrotus purpuratus
-
 8-hydroxy,9-oxo-eicosa-5Z,11Z,14Z-trienoic acid + 9-oxo-[8,12-cis]-prosta-5Z,10,14Z-trienoic acid + H2O
-
 ?
1.13.11.40 2 arachidonic acid + 2 O2 Asteroidea
-
 8-hydroxy,9-oxo-eicosa-5Z,11Z,14Z-trienoic acid + 9-oxo-[8,12-cis]-prosta-5Z,10,14Z-trienoic acid + H2O
-
 ?
1.13.11.40 2 arachidonic acid + 2 O2 Callista chione
-
 8-hydroxy,9-oxo-eicosa-5Z,11Z,14Z-trienoic acid + 9-oxo-[8,12-cis]-prosta-5Z,10,14Z-trienoic acid + H2O
-
 ?
1.13.11.40 2 arachidonic acid + 2 O2 Plexaura homomalla
-
 8-hydroxy,9-oxo-eicosa-5Z,11Z,14Z-trienoic acid + 9-oxo-[8,12-cis]-prosta-5Z,10,14Z-trienoic acid + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.40 Asteroidea
-
 starfish
-
1.13.11.40 Callista chione
-
 clam, synonym Concha fina
-
1.13.11.40 Plexaura homomalla
-
 sea whip coral
-
1.13.11.40 Strongylocentrotus purpuratus
-
 sea urchin
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.13.11.40 additional information different values obtained from cDNA and SDS-PAGE imply a post-transitional modification Plexaura homomalla

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.40
-
 Plexaura homomalla

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.40 352
-
-
 Plexaura homomalla

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.40 2 arachidonic acid + 2 O2
-
 Strongylocentrotus purpuratus 8-hydroxy,9-oxo-eicosa-5Z,11Z,14Z-trienoic acid + 9-oxo-[8,12-cis]-prosta-5Z,10,14Z-trienoic acid + H2O
-
 ?
1.13.11.40 2 arachidonic acid + 2 O2
-
 Asteroidea 8-hydroxy,9-oxo-eicosa-5Z,11Z,14Z-trienoic acid + 9-oxo-[8,12-cis]-prosta-5Z,10,14Z-trienoic acid + H2O
-
 ?
1.13.11.40 2 arachidonic acid + 2 O2
-
 Callista chione 8-hydroxy,9-oxo-eicosa-5Z,11Z,14Z-trienoic acid + 9-oxo-[8,12-cis]-prosta-5Z,10,14Z-trienoic acid + H2O
-
 ?
1.13.11.40 2 arachidonic acid + 2 O2
-
 Plexaura homomalla 8-hydroxy,9-oxo-eicosa-5Z,11Z,14Z-trienoic acid + 9-oxo-[8,12-cis]-prosta-5Z,10,14Z-trienoic acid + H2O
-
 ?
1.13.11.40 arachidonic acid + O2
-
 Strongylocentrotus purpuratus (8R)-8-hydroperoxy-5,9,11,14-eicosatetraenoic acid
-
 ?
1.13.11.40 arachidonic acid + O2
-
 Asteroidea (8R)-8-hydroperoxy-5,9,11,14-eicosatetraenoic acid
-
 ?
1.13.11.40 arachidonic acid + O2
-
 Callista chione (8R)-8-hydroperoxy-5,9,11,14-eicosatetraenoic acid
-
 ?
1.13.11.40 arachidonic acid + O2
-
 Plexaura homomalla (8R)-8-hydroperoxy-5,9,11,14-eicosatetraenoic acid
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.11.40 19.3
-
 arachidonic acid purified enzyme Plexaura homomalla
1.13.11.40 66.7
-
 arachidonic acid
-
 Plexaura homomalla