Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Schmidt, S.R.; Muller, C.R.; Kress, W.
    Murine liver homogentisate 1,2-dioxygenase. Purification to homogeneity and novel biochemical properties (1995), Eur. J. Biochem., 228, 425-430.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.13.11.5 ascorbate stabilizes, it probably protects Fe2+ from spontaneous oxidation Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.5 ascorbate strong inhibitory effect, but stabilizes Mus musculus
1.13.11.5 Co2+ incubation with Fe2+ plus Co2+ in equimolar concentrations inhibits Mus musculus
1.13.11.5 Cu2+ incubation with Fe2+ plus Cu2+ in equimolar concentrations inhibits Mus musculus
1.13.11.5 glutathione reduced glutathione slightly inhibits Mus musculus
1.13.11.5 additional information not inhibited by Ca2+ and Mn2+ Mus musculus
1.13.11.5 Ni2+ incubation with Fe2+ plus Ni2+ in equimolar concentrations inhibits Mus musculus
1.13.11.5 Zn2+ incubation with Fe2+ plus Zn2+ in equimolar concentrations inhibits Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.5 0.188
-
 homogentisate
-
 Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.13.11.5 cytosol
-
 Mus musculus 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.5 Fe2+ Fe2+ is an absolutely obligate cofactor and cannot be replaced by other divalent cations, highest enzyme activity in vitro at 2 mM Fe2+ Mus musculus
1.13.11.5 additional information Fe3+ cannot replace Fe2+ Mus musculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.5 49000
-
 3 * 49000, SDS-PAGE Mus musculus
1.13.11.5 149000
-
 gel filtration Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.5 homogentisate + O2 Mus musculus homogentisate is the natural substrate, key reaction in the catabolic pathway of aromatic amino acids, oxidative cleavage of the aromatic ring 4-maleylacetoacetate
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.5 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.5 330fold purification Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.13.11.5 liver
-
 Mus musculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.5 additional information
-
-
 Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.5 homogentisate + O2
-
 Mus musculus 4-maleylacetoacetate
-
 ir
1.13.11.5 homogentisate + O2 homogentisate is the natural substrate, key reaction in the catabolic pathway of aromatic amino acids, oxidative cleavage of the aromatic ring Mus musculus 4-maleylacetoacetate
-
 ir

Subunits

EC Number Subunits Comment Organism
1.13.11.5 trimer 3 * 49000, SDS-PAGE Mus musculus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.13.11.5 25 55 almost linear decrease of activity Mus musculus
1.13.11.5 45
-
 65% loss of activity Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.5 6.1
-
-
 Mus musculus