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Literature summary extracted from

  • Fernandez-Canon, J.M.; Penalva, M.A.
    Molecular characterization of a gene encoding a homogentisate dioxygenase from Aspergillus nidulans and identification of its human and plant homologues (1995), J. Biol. Chem., 270, 21199-21205.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.13.11.5 additional information fusion protein of homogentisate dioxygenase and glutathione S-transferase, expressed in Escherichia coli is fully active in absence of ascorbate Aspergillus nidulans

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.5 hmgA gene encoding homogentisate dioxygenase, a 448 residue polypeptide, is cloned, sequenced and characterized, gene is expressed in Escherichia coli as a fusion to glutathione S-transferase Aspergillus nidulans

Protein Variants

EC Number Protein Variants Comment Organism
1.13.11.5 additional information mutant strain biA1, methG1, argB2 with homogentisate dioxygenase gene disruption has no homogentisate dioxygenase activity and accumulates homogentisate Aspergillus nidulans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.5 Fe2+ 70% reduction of enzyme activity in crude extract in absence of Fe2+ and absolutely requirement for Fe2+ of the fusion protein of homogentisate dioxygenase and glutathione S-transferase, expressed in Escherichia coli Aspergillus nidulans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.5 50168
-
 x * 50168, calculated from the amino acid sequence Aspergillus nidulans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.5 homogentisate + O2 Aspergillus nidulans catalyzes an essential step in phenylalanine catabolism 4-maleylacetoacetate
-
 ?
1.13.11.5 homogentisate + O2 Aspergillus nidulans BiA1 catalyzes an essential step in phenylalanine catabolism 4-maleylacetoacetate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.5 Aspergillus nidulans Q00667
-
-
1.13.11.5 Aspergillus nidulans Q00667 filamentous ascomycete
-
1.13.11.5 Aspergillus nidulans BiA1 Q00667
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.5 purification of a fusion protein of homogentisate dioxygenase and glutathione S-transferase, expressed in Escherichia coli Aspergillus nidulans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.5 homogentisate + O2
-
 Aspergillus nidulans 4-maleylacetoacetate
-
 ?
1.13.11.5 homogentisate + O2 catalyzes an essential step in phenylalanine catabolism Aspergillus nidulans 4-maleylacetoacetate
-
 ?
1.13.11.5 homogentisate + O2
-
 Aspergillus nidulans BiA1 4-maleylacetoacetate
-
 ?
1.13.11.5 homogentisate + O2 catalyzes an essential step in phenylalanine catabolism Aspergillus nidulans BiA1 4-maleylacetoacetate
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.5 ? x * 50168, calculated from the amino acid sequence Aspergillus nidulans