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Literature summary extracted from

  • Sugumaran, M.; Vaidyanathan, C.S.
    Affinity chromatography of homogentisate-1,2-dioxygenase from Aspergillus niger (1978), FEMS Microbiol. Lett., 4, 343-347.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.5 2-hydroxyphenylacetate strong competitive inhibition with respect to homogentisate Aspergillus niger
1.13.11.5 3-hydroxyphenylacetate competitive inhibition with respect to homogentisate Aspergillus niger
1.13.11.5 sulfhydryl reagents strong inhibition Aspergillus niger
1.13.11.5 Superoxide dismutase inhibits the reaction indicating the involvement of superoxide anions in the dioxygenase reaction Aspergillus niger

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.5 0.6
-
 homogentisate
-
 Aspergillus niger

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.5 Fe2+ requires Fe2+ Aspergillus niger

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.5 200000
-
 gel filtration Aspergillus niger

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.5 homogentisate + O2 Aspergillus niger one of the key enzymes involved in catabolism of phenylalanine, tyrosine, phenylacetic acid and hydroxyphenylacetic acids 4-maleylacetoacetate
-
 ?
1.13.11.5 homogentisate + O2 Aspergillus niger UBC 814 one of the key enzymes involved in catabolism of phenylalanine, tyrosine, phenylacetic acid and hydroxyphenylacetic acids 4-maleylacetoacetate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.5 Aspergillus niger
-
 UBC 814
-
1.13.11.5 Aspergillus niger UBC 814
-
 UBC 814
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.5 19.2fold purification Aspergillus niger

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.5 5.17
-
-
 Aspergillus niger

Storage Stability

EC Number Storage Stability Organism
1.13.11.5 -20°C, in absence of Fe2+, about 3 days, stable Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.5 homogentisate + O2 specific for homogentisate Aspergillus niger 4-maleylacetoacetate
-
 ?
1.13.11.5 homogentisate + O2 one of the key enzymes involved in catabolism of phenylalanine, tyrosine, phenylacetic acid and hydroxyphenylacetic acids Aspergillus niger 4-maleylacetoacetate
-
 ?
1.13.11.5 homogentisate + O2 specific for homogentisate Aspergillus niger UBC 814 4-maleylacetoacetate
-
 ?
1.13.11.5 homogentisate + O2 one of the key enzymes involved in catabolism of phenylalanine, tyrosine, phenylacetic acid and hydroxyphenylacetic acids Aspergillus niger UBC 814 4-maleylacetoacetate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.5 6.4
-
-
 Aspergillus niger