Literature summary extracted from

Arciero, D.M.; Lipscomb, J.D.; Huynh, B.H.; Kent, T.A.; Munck, E.
EPR and Mossbauer studies of protocatechuate 4,5-dioxygenase. Characterization of a new Fe2+ environment (1983), J. Biol. Chem., 258, 14981-14991.

Crystallization (Commentary)

EC Number	Crystallization (Comment)	Organism
1.13.11.8	under strictly anaerobic conditions	Comamonas testosteroni

Metals/Ions

EC Number	Metals/Ions	Comment	Organism	Structure
1.13.11.8	Fe2+	characterization of Fe2+ environment with EPR and Mössbauer studies	Comamonas testosteroni
1.13.11.8	Fe2+	iron is present in active enzyme in the high-spin ferrous state	Comamonas testosteroni

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
1.13.11.8	17700	-	alpha2, beta2. 2 * 17700 + 2 * 33800, dimer of a heterodimer, SDS-PAGE	Comamonas testosteroni
1.13.11.8	33800	-	alpha2, beta2. 2 * 17700 + 2 * 33800, dimer of a heterodimer, SDS-PAGE	Comamonas testosteroni
1.13.11.8	142000	-	gel filtration	Comamonas testosteroni

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.13.11.8	Comamonas testosteroni	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
1.13.11.8	-	Comamonas testosteroni

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1.13.11.8	212	-	-	Comamonas testosteroni

Subunits

EC Number	Subunits	Comment	Organism
1.13.11.8	tetramer	alpha2, beta2. 2 * 17700 + 2 * 33800, dimer of a heterodimer, SDS-PAGE	Comamonas testosteroni

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Release 2023.1 (January 2023)