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Literature summary extracted from

  • Kosuge, T.; Heskett, M.G.; Wilson, E.E.
    Microbial synthesis and degradation of indole-3-acetic acid. I. The conversion of L-tryptophan to indole-3-acetamide by an enzyme system from Pseudomonas savastanoi (1966), J. Biol. Chem., 241, 3738-3744.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.12.3 2-mercaptoethanol weak Pseudomonas savastanoi
1.13.12.3 BaCl2 weak Pseudomonas savastanoi
1.13.12.3 CaCl2 little or no effect Pseudomonas savastanoi
1.13.12.3 CuCl2
-
Pseudomonas savastanoi
1.13.12.3 D-tryptophan weak Pseudomonas savastanoi
1.13.12.3 EDTA little or no effect Pseudomonas savastanoi
1.13.12.3 HgCl2
-
Pseudomonas savastanoi
1.13.12.3 hydroxylamine
-
Pseudomonas savastanoi
1.13.12.3 Indole-3-acetaldehyde weak Pseudomonas savastanoi
1.13.12.3 Indole-3-acetamide end product inhibition Pseudomonas savastanoi
1.13.12.3 indole-3-acetic acid
-
Pseudomonas savastanoi
1.13.12.3 iodoacetamide
-
Pseudomonas savastanoi
1.13.12.3 isoniazid
-
Pseudomonas savastanoi
1.13.12.3 K3Fe(CN)6 weak Pseudomonas savastanoi
1.13.12.3 KCN little or no effect Pseudomonas savastanoi
1.13.12.3 L-cysteine
-
Pseudomonas savastanoi
1.13.12.3 L-glutathione reduced Pseudomonas savastanoi
1.13.12.3 Quinacrine weak Pseudomonas savastanoi
1.13.12.3 Semicarbazide
-
Pseudomonas savastanoi

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.12.3 L-tryptophan + O2 Pseudomonas savastanoi specific for L-isomer of tryptophan indole-3-acetamide + CO2 + H2O
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.13.12.3 Pseudomonas savastanoi
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.12.3
-
Pseudomonas savastanoi

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.12.3 0.25
-
-
Pseudomonas savastanoi

Storage Stability

EC Number Storage Stability Organism
1.13.12.3 -10°C, protein concentration 4 mg/ml, 2 months, less than 10% loss of activity Pseudomonas savastanoi
1.13.12.3 -10°C, protein concentration less than 0.3 mg/ml, 40% loss of activity after 24 h Pseudomonas savastanoi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.12.3 5-hydroxytryptophan + O2 at 17% the rate of that for L-tryptophan Pseudomonas savastanoi 5-hydroxyindole-3-acetamide + CO2 + H2O
-
?
1.13.12.3 L-phenylalanine + O2 weak activity Pseudomonas savastanoi phenylacetamide + CO2 + H2O
-
?
1.13.12.3 L-tryptophan + O2 specific for L-isomer of tryptophan Pseudomonas savastanoi indole-3-acetamide + CO2 + H2O
-
?
1.13.12.3 L-tyrosine + O2 weak activity Pseudomonas savastanoi 4-hydroxyphenylacetamide + CO2 + H2O
-
?
1.13.12.3 additional information no activity with: indole-3-pyruvate, indole-3-acetaldehyde, alpha-methyltryptophan, indole-3-acetic acid Pseudomonas savastanoi ?
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.12.3 25
-
assay at Pseudomonas savastanoi

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.13.12.3 55
-
pH 7.4, protein concentration above 4 mg/ml, 2 min, stable, 40% loss of activity after 5 min Pseudomonas savastanoi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.12.3 7.2 9.2
-
Pseudomonas savastanoi

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.13.12.3 5.5 10 pH 5.5: about 50% of activity maximum, pH 10: about 55% of activity maximum Pseudomonas savastanoi