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Literature summary extracted from

  • Matsuda, J.; Hashimoto, T.; Yamada, Y.
    Analysis of active-site residues in hyoscyamine 6beta-hydroxylase (1997), Plant Biotechnol., 14, 51-57.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.11.11 expression in Escherichia coli as a fusion protein to maltose-binding protein Hyoscyamus niger

Protein Variants

EC Number Protein Variants Comment Organism
1.14.11.11 D219H inactive mutant enzyme Hyoscyamus niger
1.14.11.11 D219N inactive mutant enzyme Hyoscyamus niger
1.14.11.11 H217Q inactive mutant enzyme Hyoscyamus niger
1.14.11.11 H66Q mutant enzyme has 97% of the activity of the wild-type enzyme Hyoscyamus niger
1.14.11.11 S274Q inactive mutant enzyme Hyoscyamus niger

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.11 Hyoscyamus niger
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.11 L-hyoscyamine + 2-oxoglutarate + O2
-
 Hyoscyamus niger 6beta-hydroxyhyoscyamine + succinate + CO2
-
 ?