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Literature summary extracted from

  • Hashimoto, T.; Kohno, J.; Yamada, Y.
    6beta-hydroxyhyoscyamine epoxidase from cultured roots of Hyoscyamus niger (1989), Phytochemistry, 28, 1077-1082.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.20.13 catalase
-
Hyoscyamus niger

General Stability

EC Number General Stability Organism
1.14.20.13 rapid loss of enzyme activity during purification Hyoscyamus niger

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.20.13 CuSO4 100% inhibition Hyoscyamus niger
1.14.20.13 MgSO4 24% inhibition Hyoscyamus niger
1.14.20.13 MnSO4 100% inhibition Hyoscyamus niger
1.14.20.13 additional information no marked effect on enzyme activity by addition of NAD+, NADH, NADP+, NADPH, ATP + MgSO4, FAD, FMN, pyrroloquinoline quinone, acetyl-CoA, 6,7-dimethyl-5,6,7,8-tetrahydrofolate, phenazine methosulfate, 2,6-dichlorophenolindophenol, cytochrome c and H2O2 Hyoscyamus niger
1.14.20.13 Pyridine 2,4-dicarboxylate
-
Hyoscyamus niger
1.14.20.13 ZnSO4 100% inhibition Hyoscyamus niger

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.20.13 0.015
-
(6S)-6-hydroxyhyoscyamine
-
Hyoscyamus niger

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.20.13 cytoplasm
-
Hyoscyamus niger 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.20.13 Fe2+ required as co-factor Hyoscyamus niger

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.20.13 (6S)-6-hydroxyhyoscyamine + 2-oxoglutarate + O2 Hyoscyamus niger alkaloid metabolism, scopolamine is formed by oxidative transformation of hyoscyamine in several solanaceous species scopolamine + succinate + CO2
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.14.20.13 Hyoscyamus niger
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.14.20.13 requires molecular oxygen Hyoscyamus niger

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.20.13 partial, could not be separated from hyoscyamine 6beta-hydroxylase Hyoscyamus niger

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.20.13 root
-
Hyoscyamus niger
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.20.13 0.000198
-
-
Hyoscyamus niger

Storage Stability

EC Number Storage Stability Organism
1.14.20.13 -20°C, enzyme is stored in small portions until assayed Hyoscyamus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.20.13 (6S)-6-hydroxyhyoscyamine + 2-oxoglutarate + O2
-
Hyoscyamus niger scopolamine + succinate + CO2
-
?
1.14.20.13 (6S)-6-hydroxyhyoscyamine + 2-oxoglutarate + O2 alkaloid metabolism, scopolamine is formed by oxidative transformation of hyoscyamine in several solanaceous species Hyoscyamus niger scopolamine + succinate + CO2
-
?

Synonyms

EC Number Synonyms Comment Organism
1.14.20.13 hyosOH epoxidase
-
Hyoscyamus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.20.13 7.5
-
-
Hyoscyamus niger

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.20.13 2-oxoglutarate
-
Hyoscyamus niger
1.14.20.13 ascorbate
-
Hyoscyamus niger