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Literature summary extracted from

  • Wang, Q.; VanDusen, W.J.; Petrosky, C.J.; Garsky, V.M.; Stern, A.M.; Friedman, P.A.
    Bovine liver aspartyl beta-hydroxylase. Purification and characterization (1991), J. Biol. Chem., 266, 14004-14010.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.11.16 0.0083
-
 Fe2+ 56 kDa protein Bos taurus
1.14.11.16 0.0096
-
 Fe2+ 52 kDa protein Bos taurus
1.14.11.16 0.021
-
 2-oxoglutarate 56 kDa protein Bos taurus
1.14.11.16 0.022
-
 2-oxoglutarate 52 kDa protein Bos taurus
1.14.11.16 0.034
-
 first growth factor-like domain-L-aspartate substrate is of human protein S with an asparagine replacing the aspartic acid at position 18, 52 kDa protein Bos taurus
1.14.11.16 0.035
-
 first growth factor-like domain-L-aspartate substrate is of human protein S with an asparagine replacing the aspartic acid at position 18, 56 kDa protein Bos taurus
1.14.11.16 0.067
-
 first growth factor-like domain-L-aspartate substrate is of human protein S, 52 kDa protein Bos taurus
1.14.11.16 0.075
-
 first growth factor-like domain-L-aspartate substrate is of human protein S, 56 kDa protein Bos taurus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.11.16 Fe2+
-
 Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.11.16 52000
-
 SDS-PAGE, protein with lower molecular weight Bos taurus
1.14.11.16 56000
-
 SDS-PAGE, protein with higher molecular weight Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.16 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.11.16 two enzymes, one 52000 Da, the other 56000 Da Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.11.16 liver
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.11.16 0.59
-
-
 Bos taurus

Storage Stability

EC Number Storage Stability Organism
1.14.11.16 4°C, 50 mM Tris-HCl pH 7.2, 1 mg/ml bovine serum albumin, at least 8 weeks stable Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.16 first epidermal growth factor-like domain-L-aspartate + 2-oxoglutarate + O2
-
 Bos taurus first epidermal growth factor-like domain-3-hydroxy-L-aspartate + succinate + CO2
-
 r
1.14.11.16 peptide L-asparagine + 2-oxoglutarate + O2 first epidermal growth factor-like domain of human protein S with an asparagine replacing the aspartic acid at position 18 Bos taurus peptide 3-hydroxy-L-asparagine + succinate + CO2
-
 ?
1.14.11.16 peptide L-aspartate + 2-oxoglutarate + O2 first epidermal growth factor-like domain of human protein S as substrate Bos taurus peptide 3-hydroxy-L-aspartate + succinate + CO2
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.11.16 0.0167
-
 first growth factor-like domain-L-aspartate substrate is of human protein S, 56 kDa protein Bos taurus
1.14.11.16 0.0217
-
 first growth factor-like domain-L-aspartate substrate is of human protein S with an asparagine replacing the aspartic acid at position 18, 56 kDa protein Bos taurus
1.14.11.16 0.025
-
 first growth factor-like domain-L-aspartate substrate is of human protein S, 52 kDa protein Bos taurus
1.14.11.16 0.0317
-
 first growth factor-like domain-L-aspartate substrate is of human protein S with an asparagine replacing the aspartic acid at position 18, 52 kDa protein Bos taurus