BRENDA - Enzyme Database

A fully active catalytic domain of bovine aspartyl (asparaginyl) beta-hydroxylase expressed in Escherichia coli: characterization and evidence for the identification of an active-site region in vertebrate alpha-ketoglutarate-dependent dioxygenases

Jia, S.; McGinnis, K.; VanDusen, W.J.; Burke, C.J.; Kuo, A.; Griffin, P.R.; Sardana, M.K.; Elliston, K.O.; Stern, A.M.; Friedman, P.A.; Proc. Natl. Acad. Sci. USA 91, 7227-7231 (1994)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.14.11.16
expressed in wild-type Escherichia coli and in Escherichia coli BL21
Bos taurus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.14.11.16
iodoacetamide
at 1 mM less than 5% activity, 2-oxoglutarate and EDTA protects
Bos taurus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.11.16
0.019
-
first epidermal growth factor-like domain
substrate is of human protein S with an asparagine replacing the aspartic acid at position 18, native enzyme
Bos taurus
1.14.11.16
0.024
-
first epidermal growth factor-like domain
substrate is of human protein S with an asparagine replacing the aspartic acid at position 18, recombinant enzyme
Bos taurus
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.14.11.16
Fe2+
-
Bos taurus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.14.11.16
50700
-
sedimentation equilibrium
Bos taurus
1.14.11.16
51030
-
MALDI-TOF
Bos taurus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.11.16
Bos taurus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.14.11.16
-
Bos taurus
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.14.11.16
14
-
-
Bos taurus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.11.16
peptide L-asparagine + 2-oxoglutarate + O2
first epidermal growth factor-like domain of human protein S with an asparagine replacing the aspartic acid at position 18
439256
Bos taurus
peptide 3-hydroxy-L-asparagine + succinate + CO2
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.14.11.16
monomer
1 * 50700, sedimentation equilibrium
Bos taurus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.11.16
expressed in wild-type Escherichia coli and in Escherichia coli BL21
Bos taurus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.14.11.16
iodoacetamide
at 1 mM less than 5% activity, 2-oxoglutarate and EDTA protects
Bos taurus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.11.16
0.019
-
first epidermal growth factor-like domain
substrate is of human protein S with an asparagine replacing the aspartic acid at position 18, native enzyme
Bos taurus
1.14.11.16
0.024
-
first epidermal growth factor-like domain
substrate is of human protein S with an asparagine replacing the aspartic acid at position 18, recombinant enzyme
Bos taurus
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.14.11.16
Fe2+
-
Bos taurus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.14.11.16
50700
-
sedimentation equilibrium
Bos taurus
1.14.11.16
51030
-
MALDI-TOF
Bos taurus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.11.16
-
Bos taurus
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.14.11.16
14
-
-
Bos taurus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.11.16
peptide L-asparagine + 2-oxoglutarate + O2
first epidermal growth factor-like domain of human protein S with an asparagine replacing the aspartic acid at position 18
439256
Bos taurus
peptide 3-hydroxy-L-asparagine + succinate + CO2
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.14.11.16
monomer
1 * 50700, sedimentation equilibrium
Bos taurus