BRENDA - Enzyme Database

Cofactor and substrate requirements of collagen proline hydroxylase

Hutton, J.J.; Tappel, A.L.; Udenfried, S.; Arch. Biochem. Biophys. 118, 231-240 (1967)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.14.11.2
1,10-phenanthroline
91% inhibition at 0.02 mM
Rattus norvegicus
1.14.11.2
2,2'-dipyridyl
-
Gallus gallus
1.14.11.2
2,2'-dipyridyl
87% inhibition at 0.02 mM
Rattus norvegicus
1.14.11.2
antimycin A
18% inhibition at 0.02 mM
Rattus norvegicus
1.14.11.2
Cupferron
28% inhibition at 0.02 mM
Rattus norvegicus
1.14.11.2
diethyldithiocarbamate
10% inhibition at 0.02 mM
Rattus norvegicus
1.14.11.2
EDTA
-
Gallus gallus
1.14.11.2
EDTA
64% inhibition at 0.02 mM
Rattus norvegicus
1.14.11.2
gelatin
commercial, inhibitor
Cavia porcellus
1.14.11.2
gelatin
commercial, inhibitor
Gallus gallus
1.14.11.2
gelatin
commercial, inhibitor
Rattus norvegicus
1.14.11.2
Ketomalonate
100% inhibition at 1 mM, acts by chelating ferrous ion rather than by competing with alpha-ketoglutarate
Cavia porcellus
1.14.11.2
Ketomalonate
100% inhibition at 1 mM, acts by chelating ferrous ion rather than by competing with alpha-ketoglutarate
Gallus gallus
1.14.11.2
Ketomalonate
100% inhibition at 1 mM, acts by chelating ferrous ion rather than by competing with alpha-ketoglutarate
Rattus norvegicus
1.14.11.2
malonate
19% inhibition at 1 mM
Cavia porcellus
1.14.11.2
malonate
19% inhibition at 1 mM
Gallus gallus
1.14.11.2
malonate
19% inhibition at 1 mM
Rattus norvegicus
1.14.11.2
oxalate
34% inhibition at 1 mM
Cavia porcellus
1.14.11.2
oxalate
34% inhibition at 1 mM
Gallus gallus
1.14.11.2
oxalate
34% inhibition at 1 mM
Rattus norvegicus
1.14.11.2
propyl gallate
100% inhibition at 2 mM
Cavia porcellus
1.14.11.2
propyl gallate
100% inhibition at 2 mM
Gallus gallus
1.14.11.2
propyl gallate
100% inhibition at 2 mM
Rattus norvegicus
1.14.11.2
succinate
51% inhibition at 1 mM
Cavia porcellus
1.14.11.2
succinate
51% inhibition at 1 mM
Gallus gallus
1.14.11.2
succinate
51% inhibition at 1 mM
Rattus norvegicus
1.14.11.2
trifluorothienylbutanedione
48% inhibition at 0.05 mM
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.11.2
0.03
-
O2
-
Cavia porcellus
1.14.11.2
0.03
-
O2
-
Gallus gallus
1.14.11.2
0.03
-
O2
-
Rattus norvegicus
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.14.11.2
Fe2+
no compound can replace Fe2+, Km: 0.03 mM
Cavia porcellus
1.14.11.2
Fe2+
no compound can replace Fe2+, Km: 0.03 mM
Gallus gallus
1.14.11.2
Fe2+
no compound can replace Fe2+, Km: 0.03 mM
Rattus norvegicus
1.14.11.2
Mg2+
3% relative activity with respect to Fe2+
Gallus gallus
1.14.11.2
Mn2+
10% relative activity with respect to Fe2+
Gallus gallus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.11.2
Cavia porcellus
-
-
-
1.14.11.2
Gallus gallus
-
-
-
1.14.11.2
Rattus norvegicus
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.11.2
embryo
-
Gallus gallus
-
1.14.11.2
skin
-
Gallus gallus
-
1.14.11.2
skin
fetus
Rattus norvegicus
-
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.14.11.2
30
-
-
Cavia porcellus
1.14.11.2
30
-
-
Gallus gallus
1.14.11.2
30
-
-
Rattus norvegicus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.14.11.2
2-oxoglutarate
no compound can replace alpha-ketoglutarate
Cavia porcellus
1.14.11.2
2-oxoglutarate
no compound can replace alpha-ketoglutarate
Gallus gallus
1.14.11.2
2-oxoglutarate
no compound can replace alpha-ketoglutarate
Rattus norvegicus
1.14.11.2
ascorbate
Km: 0.2-1 mM, dependent on the concentrations of alpha-ketoglutarate and Fe2+, reduced pteridines can partially replace ascorbate; requirement
Cavia porcellus
1.14.11.2
ascorbate
Km: 0.2-1 mM, dependent on the concentrations of alpha-ketoglutarate and Fe2+, reduced pteridines can partially replace ascorbate; requirement
Gallus gallus
1.14.11.2
ascorbate
Km: 0.2-1 mM, dependent on the concentrations of alpha-ketoglutarate and Fe2+, reduced pteridines can partially replace ascorbate; requirement
Rattus norvegicus
1.14.11.2
D-isoascorbate
can replace ascorbate
Cavia porcellus
1.14.11.2
D-isoascorbate
can replace ascorbate
Gallus gallus
1.14.11.2
D-isoascorbate
can replace ascorbate
Rattus norvegicus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.14.11.2
2-oxoglutarate
no compound can replace alpha-ketoglutarate
Cavia porcellus
1.14.11.2
2-oxoglutarate
no compound can replace alpha-ketoglutarate
Gallus gallus
1.14.11.2
2-oxoglutarate
no compound can replace alpha-ketoglutarate
Rattus norvegicus
1.14.11.2
ascorbate
Km: 0.2-1 mM, dependent on the concentrations of alpha-ketoglutarate and Fe2+, reduced pteridines can partially replace ascorbate; requirement
Cavia porcellus
1.14.11.2
ascorbate
Km: 0.2-1 mM, dependent on the concentrations of alpha-ketoglutarate and Fe2+, reduced pteridines can partially replace ascorbate; requirement
Gallus gallus
1.14.11.2
ascorbate
Km: 0.2-1 mM, dependent on the concentrations of alpha-ketoglutarate and Fe2+, reduced pteridines can partially replace ascorbate; requirement
Rattus norvegicus
1.14.11.2
D-isoascorbate
can replace ascorbate
Cavia porcellus
1.14.11.2
D-isoascorbate
can replace ascorbate
Gallus gallus
1.14.11.2
D-isoascorbate
can replace ascorbate
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.14.11.2
1,10-phenanthroline
91% inhibition at 0.02 mM
Rattus norvegicus
1.14.11.2
2,2'-dipyridyl
-
Gallus gallus
1.14.11.2
2,2'-dipyridyl
87% inhibition at 0.02 mM
Rattus norvegicus
1.14.11.2
antimycin A
18% inhibition at 0.02 mM
Rattus norvegicus
1.14.11.2
Cupferron
28% inhibition at 0.02 mM
Rattus norvegicus
1.14.11.2
diethyldithiocarbamate
10% inhibition at 0.02 mM
Rattus norvegicus
1.14.11.2
EDTA
-
Gallus gallus
1.14.11.2
EDTA
64% inhibition at 0.02 mM
Rattus norvegicus
1.14.11.2
gelatin
commercial, inhibitor
Cavia porcellus
1.14.11.2
gelatin
commercial, inhibitor
Gallus gallus
1.14.11.2
gelatin
commercial, inhibitor
Rattus norvegicus
1.14.11.2
Ketomalonate
100% inhibition at 1 mM, acts by chelating ferrous ion rather than by competing with alpha-ketoglutarate
Cavia porcellus
1.14.11.2
Ketomalonate
100% inhibition at 1 mM, acts by chelating ferrous ion rather than by competing with alpha-ketoglutarate
Gallus gallus
1.14.11.2
Ketomalonate
100% inhibition at 1 mM, acts by chelating ferrous ion rather than by competing with alpha-ketoglutarate
Rattus norvegicus
1.14.11.2
malonate
19% inhibition at 1 mM
Cavia porcellus
1.14.11.2
malonate
19% inhibition at 1 mM
Gallus gallus
1.14.11.2
malonate
19% inhibition at 1 mM
Rattus norvegicus
1.14.11.2
oxalate
34% inhibition at 1 mM
Cavia porcellus
1.14.11.2
oxalate
34% inhibition at 1 mM
Gallus gallus
1.14.11.2
oxalate
34% inhibition at 1 mM
Rattus norvegicus
1.14.11.2
propyl gallate
100% inhibition at 2 mM
Cavia porcellus
1.14.11.2
propyl gallate
100% inhibition at 2 mM
Gallus gallus
1.14.11.2
propyl gallate
100% inhibition at 2 mM
Rattus norvegicus
1.14.11.2
succinate
51% inhibition at 1 mM
Cavia porcellus
1.14.11.2
succinate
51% inhibition at 1 mM
Gallus gallus
1.14.11.2
succinate
51% inhibition at 1 mM
Rattus norvegicus
1.14.11.2
trifluorothienylbutanedione
48% inhibition at 0.05 mM
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.11.2
0.03
-
O2
-
Cavia porcellus
1.14.11.2
0.03
-
O2
-
Gallus gallus
1.14.11.2
0.03
-
O2
-
Rattus norvegicus
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.14.11.2
Fe2+
no compound can replace Fe2+, Km: 0.03 mM
Cavia porcellus
1.14.11.2
Fe2+
no compound can replace Fe2+, Km: 0.03 mM
Gallus gallus
1.14.11.2
Fe2+
no compound can replace Fe2+, Km: 0.03 mM
Rattus norvegicus
1.14.11.2
Mg2+
3% relative activity with respect to Fe2+
Gallus gallus
1.14.11.2
Mn2+
10% relative activity with respect to Fe2+
Gallus gallus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.11.2
embryo
-
Gallus gallus
-
1.14.11.2
skin
-
Gallus gallus
-
1.14.11.2
skin
fetus
Rattus norvegicus
-
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.14.11.2
30
-
-
Cavia porcellus
1.14.11.2
30
-
-
Gallus gallus
1.14.11.2
30
-
-
Rattus norvegicus