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Literature summary extracted from

  • Wang, C.; Risteli, M.; Heikkinen, J.; Hussa, A.K.; Uitto, L.; Myllyla, R.
    Identification of amino acids important for the catalytic activity of the collagen glucosyltransferase associated with the multifunctional lysyl hydroxylase 3 (LH3) (2002), J. Biol. Chem., 277, 18568-18573.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.11.4 expression in Escherichia coli, isoform 3 gene product possesses the collagen glycosyltransferase activity, but not isoform 1 and 2 Caenorhabditis elegans
2.4.1.66 cDNA sequence of GGT/LH3 Homo sapiens
2.4.1.66 LH cDNA with GGT activity is expressed in Escherichia coli XL1-Blue, cDNA sequence of GGT/LH Caenorhabditis elegans
2.4.1.66 LH3 cDNA with GGT activity is cloned and expressed in Sf9 insect cells and in Escherichia coli XL1-Blue Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.14.11.4 C144I isoform 3, reduces glycosyltransferase activity Caenorhabditis elegans
1.14.11.4 L208I isoform 3, reduces glycosyltransferase activity Caenorhabditis elegans
2.4.1.66 A361G mutant with reduced GGT activity Caenorhabditis elegans
2.4.1.66 A380V mutant with reduced GGT activity Caenorhabditis elegans
2.4.1.66 A453I mutant with a remarkably reduced GGT activity Caenorhabditis elegans
2.4.1.66 A464I no effect on GGT activity Homo sapiens
2.4.1.66 C132I mutant with dramatically reduced GGT activity Caenorhabditis elegans
2.4.1.66 C144I mutant with dramatically reduced GGT activity Homo sapiens
2.4.1.66 D331V little effect on GGT activity Caenorhabditis elegans
2.4.1.66 D392A little effect on GGT activity Homo sapiens
2.4.1.66 E371T mutant with reduced GGT activity Caenorhabditis elegans
2.4.1.66 E547K mutant with increased GGT activity Caenorhabditis elegans
2.4.1.66 F131L mutant with markedly reduced GGT activity Caenorhabditis elegans
2.4.1.66 F143L mutant with reduced GGT activity Homo sapiens
2.4.1.66 F372Y little effect on GGT activity Caenorhabditis elegans
2.4.1.66 H165S mutant with reduced GGT activity Caenorhabditis elegans
2.4.1.66 K482A mutant with increased GGT activity Caenorhabditis elegans
2.4.1.66 K674V no effect on GGT activity Caenorhabditis elegans
2.4.1.66 L124V mutant with 40% inhibited GGT activity Caenorhabditis elegans
2.4.1.66 L125V mutant with 70% inhibited GGT activity Caenorhabditis elegans
2.4.1.66 L136V mutant with increased GGT activity Homo sapiens
2.4.1.66 L137V mutant with increased GGT activity Homo sapiens
2.4.1.66 L192K mutant with reduced GGT activity Caenorhabditis elegans
2.4.1.66 L196I mutant with markedly reduced GGT activity Caenorhabditis elegans
2.4.1.66 L208I mutant with markedly reduced GGT activity Homo sapiens
2.4.1.66 L42K mutant with increased GGT activity Caenorhabditis elegans
2.4.1.66 L491M mutant with little increased GGT activity Caenorhabditis elegans
2.4.1.66 L562I mutant with increased GGT activity Caenorhabditis elegans
2.4.1.66 L591I mutant with increased GGT activity Caenorhabditis elegans
2.4.1.66 M406L mutant with reduced GGT activity Caenorhabditis elegans
2.4.1.66 M642L mutant with increased GGT activity Caenorhabditis elegans
2.4.1.66 M650L no effect on GGT activity Homo sapiens
2.4.1.66 M724A little effect on GGT activity Caenorhabditis elegans
2.4.1.66 additional information mutagenesis of aspartates of the DXD-like motif in LH3 eliminates GGT activity Homo sapiens
2.4.1.66 P591N no effect on GGT activity Caenorhabditis elegans
2.4.1.66 Q613E mutant with little increased GGT activity Caenorhabditis elegans
2.4.1.66 S204C mutant with reduced GGT activity Caenorhabditis elegans
2.4.1.66 V622L no effect on GGT activity Caenorhabditis elegans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.66 soluble
-
Homo sapiens
-
-
2.4.1.66 soluble
-
Caenorhabditis elegans
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.66 Mn2+
-
Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.66 UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen Homo sapiens posttranslational modification of collagen UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen
-
?
2.4.1.66 UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen Caenorhabditis elegans posttranslational modification of collagen UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.4 Caenorhabditis elegans
-
-
-
2.4.1.66 Caenorhabditis elegans
-
-
-
2.4.1.66 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.14.11.4 side-chain modification
-
Caenorhabditis elegans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.4 peptidyl-L-lysine + 2-oxoglutarate + O2
-
Caenorhabditis elegans peptidyl-5-hydroxy-L-lysine + succinate + CO2 hydroxy-L-lysine ?
2.4.1.66 additional information the amino acids important for the GGT activity are located in the N-terminal part of LH3/GGT protein separate from the C-terminal LH active site Homo sapiens ?
-
?
2.4.1.66 additional information lysyl hydroxylase and collagen glucosyltransferase activities are associated with the same gene product, the amino acids important for the GGT activity are located in the N-terminal part of LH/GGT protein separate from the C-terminal LH active site, C-132 and L-196 are important for the catalytic activity of GGT Caenorhabditis elegans ?
-
?
2.4.1.66 additional information multifunctional lysyl hydroxylase isoform 3, LH3 protein, possesses lysyl hydroxylase and collagen glucosyltransferase activities Homo sapiens ?
-
?
2.4.1.66 additional information C-144 and L-208 are important for the catalytic activity of GGT, DXD-like motif is required for activity, no GGT activity in LH1 and LH2 Homo sapiens ?
-
?
2.4.1.66 UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen calf skin gelatin Homo sapiens UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen
-
?
2.4.1.66 UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen calf skin gelatin Caenorhabditis elegans UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen
-
?
2.4.1.66 UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen posttranslational modification of collagen Homo sapiens UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen
-
?
2.4.1.66 UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen posttranslational modification of collagen Caenorhabditis elegans UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen
-
?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.66 More GGT activity is associated with lysyl hydroxylase isoform 3, LH3, EC 1.14.11.4 Homo sapiens
2.4.1.66 More GGT activity is associated with lysyl hydroxylase, LH Caenorhabditis elegans
2.4.1.66 More cf. EC 2.4.1.50 Homo sapiens
2.4.1.66 More cf. EC 2.4.1.50 Caenorhabditis elegans