EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.11.4 | expression in Escherichia coli, isoform 3 gene product possesses the collagen glycosyltransferase activity, but not isoform 1 and 2 | Caenorhabditis elegans |
2.4.1.66 | cDNA sequence of GGT/LH3 | Homo sapiens |
2.4.1.66 | LH cDNA with GGT activity is expressed in Escherichia coli XL1-Blue, cDNA sequence of GGT/LH | Caenorhabditis elegans |
2.4.1.66 | LH3 cDNA with GGT activity is cloned and expressed in Sf9 insect cells and in Escherichia coli XL1-Blue | Homo sapiens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.14.11.4 | C144I | isoform 3, reduces glycosyltransferase activity | Caenorhabditis elegans |
1.14.11.4 | L208I | isoform 3, reduces glycosyltransferase activity | Caenorhabditis elegans |
2.4.1.66 | A361G | mutant with reduced GGT activity | Caenorhabditis elegans |
2.4.1.66 | A380V | mutant with reduced GGT activity | Caenorhabditis elegans |
2.4.1.66 | A453I | mutant with a remarkably reduced GGT activity | Caenorhabditis elegans |
2.4.1.66 | A464I | no effect on GGT activity | Homo sapiens |
2.4.1.66 | C132I | mutant with dramatically reduced GGT activity | Caenorhabditis elegans |
2.4.1.66 | C144I | mutant with dramatically reduced GGT activity | Homo sapiens |
2.4.1.66 | D331V | little effect on GGT activity | Caenorhabditis elegans |
2.4.1.66 | D392A | little effect on GGT activity | Homo sapiens |
2.4.1.66 | E371T | mutant with reduced GGT activity | Caenorhabditis elegans |
2.4.1.66 | E547K | mutant with increased GGT activity | Caenorhabditis elegans |
2.4.1.66 | F131L | mutant with markedly reduced GGT activity | Caenorhabditis elegans |
2.4.1.66 | F143L | mutant with reduced GGT activity | Homo sapiens |
2.4.1.66 | F372Y | little effect on GGT activity | Caenorhabditis elegans |
2.4.1.66 | H165S | mutant with reduced GGT activity | Caenorhabditis elegans |
2.4.1.66 | K482A | mutant with increased GGT activity | Caenorhabditis elegans |
2.4.1.66 | K674V | no effect on GGT activity | Caenorhabditis elegans |
2.4.1.66 | L124V | mutant with 40% inhibited GGT activity | Caenorhabditis elegans |
2.4.1.66 | L125V | mutant with 70% inhibited GGT activity | Caenorhabditis elegans |
2.4.1.66 | L136V | mutant with increased GGT activity | Homo sapiens |
2.4.1.66 | L137V | mutant with increased GGT activity | Homo sapiens |
2.4.1.66 | L192K | mutant with reduced GGT activity | Caenorhabditis elegans |
2.4.1.66 | L196I | mutant with markedly reduced GGT activity | Caenorhabditis elegans |
2.4.1.66 | L208I | mutant with markedly reduced GGT activity | Homo sapiens |
2.4.1.66 | L42K | mutant with increased GGT activity | Caenorhabditis elegans |
2.4.1.66 | L491M | mutant with little increased GGT activity | Caenorhabditis elegans |
2.4.1.66 | L562I | mutant with increased GGT activity | Caenorhabditis elegans |
2.4.1.66 | L591I | mutant with increased GGT activity | Caenorhabditis elegans |
2.4.1.66 | M406L | mutant with reduced GGT activity | Caenorhabditis elegans |
2.4.1.66 | M642L | mutant with increased GGT activity | Caenorhabditis elegans |
2.4.1.66 | M650L | no effect on GGT activity | Homo sapiens |
2.4.1.66 | M724A | little effect on GGT activity | Caenorhabditis elegans |
2.4.1.66 | additional information | mutagenesis of aspartates of the DXD-like motif in LH3 eliminates GGT activity | Homo sapiens |
2.4.1.66 | P591N | no effect on GGT activity | Caenorhabditis elegans |
2.4.1.66 | Q613E | mutant with little increased GGT activity | Caenorhabditis elegans |
2.4.1.66 | S204C | mutant with reduced GGT activity | Caenorhabditis elegans |
2.4.1.66 | V622L | no effect on GGT activity | Caenorhabditis elegans |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.4.1.66 | soluble | - |
Homo sapiens | - |
- |
2.4.1.66 | soluble | - |
Caenorhabditis elegans | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.4.1.66 | Mn2+ | - |
Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.66 | UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen | Homo sapiens | posttranslational modification of collagen | UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen | - |
? | |
2.4.1.66 | UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen | Caenorhabditis elegans | posttranslational modification of collagen | UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.11.4 | Caenorhabditis elegans | - |
- |
- |
2.4.1.66 | Caenorhabditis elegans | - |
- |
- |
2.4.1.66 | Homo sapiens | - |
- |
- |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
1.14.11.4 | side-chain modification | - |
Caenorhabditis elegans |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.11.4 | peptidyl-L-lysine + 2-oxoglutarate + O2 | - |
Caenorhabditis elegans | peptidyl-5-hydroxy-L-lysine + succinate + CO2 | hydroxy-L-lysine | ? | |
2.4.1.66 | additional information | the amino acids important for the GGT activity are located in the N-terminal part of LH3/GGT protein separate from the C-terminal LH active site | Homo sapiens | ? | - |
? | |
2.4.1.66 | additional information | lysyl hydroxylase and collagen glucosyltransferase activities are associated with the same gene product, the amino acids important for the GGT activity are located in the N-terminal part of LH/GGT protein separate from the C-terminal LH active site, C-132 and L-196 are important for the catalytic activity of GGT | Caenorhabditis elegans | ? | - |
? | |
2.4.1.66 | additional information | multifunctional lysyl hydroxylase isoform 3, LH3 protein, possesses lysyl hydroxylase and collagen glucosyltransferase activities | Homo sapiens | ? | - |
? | |
2.4.1.66 | additional information | C-144 and L-208 are important for the catalytic activity of GGT, DXD-like motif is required for activity, no GGT activity in LH1 and LH2 | Homo sapiens | ? | - |
? | |
2.4.1.66 | UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen | calf skin gelatin | Homo sapiens | UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen | - |
? | |
2.4.1.66 | UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen | calf skin gelatin | Caenorhabditis elegans | UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen | - |
? | |
2.4.1.66 | UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen | posttranslational modification of collagen | Homo sapiens | UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen | - |
? | |
2.4.1.66 | UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen | posttranslational modification of collagen | Caenorhabditis elegans | UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.1.66 | More | GGT activity is associated with lysyl hydroxylase isoform 3, LH3, EC 1.14.11.4 | Homo sapiens |
2.4.1.66 | More | GGT activity is associated with lysyl hydroxylase, LH | Caenorhabditis elegans |
2.4.1.66 | More | cf. EC 2.4.1.50 | Homo sapiens |
2.4.1.66 | More | cf. EC 2.4.1.50 | Caenorhabditis elegans |