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Literature summary extracted from

  • Passoja, K.; Rautavuoma, K.; Ala-Kokko, L.; Kosonen, T.; Kivirikko, K.I.
    Cloning and characterization of a third human lysyl hydroxylase isoform (1998), Proc. Natl. Acad. Sci. USA, 95, 10482-10486.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.14.11.4 medicine deficiency in enzyme activity causes the Ehler-Danlos syndrome type 6 Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.11.4 expression in insect cells using a baculovirus vector Homo sapiens
1.14.11.4 expression in various human tissues Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.11.4 0.1
-
 2-oxoglutarate lysyl hydroxylases 1 and 3 Homo sapiens
1.14.11.4 0.3
-
 ascorbate lysyl hydroxylase 3 Homo sapiens
1.14.11.4 0.35
-
 ascorbate lysyl hydroxylase 1 Homo sapiens
1.14.11.4 0.5
-
 Ala-Arg-Gly-Ile-Lys-Gly-Ile-Arg-Gly-Phe-Ser-Gly lysyl hydroxylase 1 Homo sapiens
1.14.11.4 0.6
-
 Ala-Arg-Gly-Ile-Lys-Gly-Ile-Arg-Gly-Phe-Ser-Gly lysyl hydroxylase 3 Homo sapiens
1.14.11.4 0.7
-
 (Ile-Lys-Gly)3 lysyl hydroxylase 1 Homo sapiens
1.14.11.4 0.8
-
 (Ile-Lys-Gly)3 lysyl hydroxylase 3 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.11.4 82380
-
 2 * 82380, calculation from amino acid sequence, recombinant enzyme: isoform 3 Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.11.4 collagen + 2-oxoglutarate + O2 Homo sapiens enzyme required during collagen biosynthesis 5-hydroxylysyl-collagen + succinate + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.4 Homo sapiens O60568
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.14.11.4 side-chain modification hydroxylation of lysyl residue in -X-Lys-Gly Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.11.4 additional information existence of isoforms Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.4 (Ile-Lys-Gly)3 + 2-oxoglutarate + O2
-
 Homo sapiens (Ile-5-hydroxylyseine-Gly)3 + succinate + CO2
-
 ?
1.14.11.4 2-oxoglutarate + O2 + ascorbate
-
 Homo sapiens succinate + CO2 + dehydroascorbate + H2O
-
 ?
1.14.11.4 Ala-Arg-Gly-Ile-Lys-Gly-Ile-Arg-Gly-Phe-Ser-Gly + 2-oxoglutarate + O2
-
 Homo sapiens Ala-Arg-Gly-Ile-5-hydroxylysine-Gly-Ile-Arg-Gly-Phe-Ser-Gly + succinate + CO2
-
 ?
1.14.11.4 collagen + 2-oxoglutarate + O2 enzyme required during collagen biosynthesis Homo sapiens 5-hydroxylysyl-collagen + succinate + CO2
-
 ?
1.14.11.4 peptidyl-L-lysine + 2-oxoglutarate + O2 synthetic peptides Homo sapiens peptidyl-5-hydroxy-L-lysine + succinate + CO2 hydroxy-L-lysine ?

Subunits

EC Number Subunits Comment Organism
1.14.11.4 homodimer 2 * 82380, calculation from amino acid sequence, recombinant enzyme: isoform 3 Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
1.14.11.4 lysyl hydroxylase lysyl hydroxylase 3 Homo sapiens