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Literature summary extracted from

  • Kivirikko, K.I.; Prockop, D.J.
    Partial purification and characterization of protocollagen lysine hydroxylase from chick embryos (1972), Biochim. Biophys. Acta, 258, 366-379.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.11.4 bovine serum albumin
-
 Gallus gallus
1.14.11.4 catalase
-
 Gallus gallus
1.14.11.4 dithiothreitol
-
 Gallus gallus

General Stability

EC Number General Stability Organism
1.14.11.4 enzyme formes aggregates in low ionic strength buffer Gallus gallus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.11.4 p-mercuribenzoate
-
 Gallus gallus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.11.4 0.05
-
 ascorbate
-
 Gallus gallus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.11.4 Fe2+ required, Km: 0.001-0.005 mM Gallus gallus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.11.4 200000
-
 gel filtration Gallus gallus
1.14.11.4 550000
-
 gel filtration, peak 2 Gallus gallus

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.4 Gallus gallus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.11.4
-
 Gallus gallus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.11.4 embryo
-
 Gallus gallus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.11.4 additional information
-
-
 Gallus gallus

Storage Stability

EC Number Storage Stability Organism
1.14.11.4 0-4°C Gallus gallus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.4 2-oxoglutarate + O2 + ascorbate
-
 Gallus gallus succinate + CO2 + dehydroascorbate + H2O
-
 ?
1.14.11.4 peptidyl-L-lysine + 2-oxoglutarate + O2 synthetic peptides Gallus gallus peptidyl-5-hydroxy-L-lysine + succinate + CO2 hydroxy-L-lysine ?
1.14.11.4 peptidyl-L-lysine + 2-oxoglutarate + O2 protocollagen Gallus gallus peptidyl-5-hydroxy-L-lysine + succinate + CO2 hydroxy-L-lysine ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.11.4 7.4
-
-
 Gallus gallus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.11.4 ascorbate required Gallus gallus