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Literature summary extracted from

  • Lukacin, R.; Groning, I.; Pieper, U.; Matern, U.
    Site-directed mutagenesis of the active site serine290 in flavanone 3beta-hydroxylase from Petunia hybrida (2000), Eur. J. Biochem., 267, 853-860.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.11.9 wild-type and mutant enzymes, expression in Escherichia coli Petunia x hybrida

Protein Variants

EC Number Protein Variants Comment Organism
1.14.11.9 S290A activity is reduced to 8% of that of the wild-type enzyme Petunia x hybrida
1.14.11.9 S290T activity is reduced to 20% of that of the wild-type enzyme Petunia x hybrida
1.14.11.9 S290V activity is reduced to 1% of that of the wild-type enzyme Petunia x hybrida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.11.9 0.0019
-
2-oxoglutarate wild-type enzyme Petunia x hybrida
1.14.11.9 0.0256
-
2-oxoglutarate mutant enzyme S290T Petunia x hybrida
1.14.11.9 0.08
-
2-oxoglutarate mutant enzyme S290A Petunia x hybrida
1.14.11.9 0.1896
-
2-oxoglutarate mutant enzyme S290V Petunia x hybrida

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.11.9 soluble
-
Petunia x hybrida
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.11.9 naringenin + 2-oxoglutarate + O2 Petunia x hybrida enzyme is involved in the biosynthesis of flavonoids, catechins, and anthocyanidins (2R,3R)-dihydrokaempferol + succinate + CO2
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.9 Petunia x hybrida
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.11.9 wild-type and mutant enzymes Petunia x hybrida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.9 naringenin + 2-oxoglutarate + O2 enzyme is involved in the biosynthesis of flavonoids, catechins, and anthocyanidins Petunia x hybrida (2R,3R)-dihydrokaempferol + succinate + CO2
-
?