EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.11.9 | wild-type and mutant enzymes, expression in Escherichia coli | Petunia x hybrida |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.14.11.9 | S290A | activity is reduced to 8% of that of the wild-type enzyme | Petunia x hybrida |
1.14.11.9 | S290T | activity is reduced to 20% of that of the wild-type enzyme | Petunia x hybrida |
1.14.11.9 | S290V | activity is reduced to 1% of that of the wild-type enzyme | Petunia x hybrida |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.11.9 | 0.0019 | - |
2-oxoglutarate | wild-type enzyme | Petunia x hybrida | |
1.14.11.9 | 0.0256 | - |
2-oxoglutarate | mutant enzyme S290T | Petunia x hybrida | |
1.14.11.9 | 0.08 | - |
2-oxoglutarate | mutant enzyme S290A | Petunia x hybrida | |
1.14.11.9 | 0.1896 | - |
2-oxoglutarate | mutant enzyme S290V | Petunia x hybrida |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.14.11.9 | soluble | - |
Petunia x hybrida | - |
- |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.11.9 | naringenin + 2-oxoglutarate + O2 | Petunia x hybrida | enzyme is involved in the biosynthesis of flavonoids, catechins, and anthocyanidins | (2R,3R)-dihydrokaempferol + succinate + CO2 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.11.9 | Petunia x hybrida | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.11.9 | wild-type and mutant enzymes | Petunia x hybrida |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.11.9 | naringenin + 2-oxoglutarate + O2 | enzyme is involved in the biosynthesis of flavonoids, catechins, and anthocyanidins | Petunia x hybrida | (2R,3R)-dihydrokaempferol + succinate + CO2 | - |
? |