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Literature summary extracted from

  • Britsch, L.
    Purification and characterization of flavone synthase I, a 2-oxoglutarate-dependent desaturase (1990), Arch. Biochem. Biophys., 282, 152-160.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.11.9 ascorbate required Petroselinum crispum
1.14.20.5 ascorbate required for full activity, can not be replaced as a reductant by 2-mercaptoethanol or dithiothreitol Petroselinum crispum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.11.9 Cu2+ in presence of 0.01 mM Fe2+ Petroselinum crispum
1.14.11.9 Pyridine-2,4-dicarboxylate most potent competitive inhibitor Petroselinum crispum
1.14.11.9 Zn2+ in presence of 0.01 mM Fe2+ Petroselinum crispum
1.14.20.5 2,4-pyridinedicarboxylate potent competitive Petroselinum crispum
1.14.20.5 Cu2+ 0.02 mM, complete inhibition in presence of 0.01 mM Fe2+ Petroselinum crispum
1.14.20.5 prunin competitive with respect to (2S)-naringenin Petroselinum crispum
1.14.20.5 Zn2+ 0.02 mM, complete inhibition in presence of 0.01 mM Fe2+ Petroselinum crispum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.11.9 0.005
-
 (2S)-naringenin
-
 Petroselinum crispum
1.14.11.9 0.008
-
 (2S)-eriodictyol
-
 Petroselinum crispum
1.14.11.9 0.016
-
 2-oxoglutarate reaction with naringenin Petroselinum crispum
1.14.20.5 0.005
-
 2S-naringenin
-
 Petroselinum crispum
1.14.20.5 0.008
-
 2S-eriodictyol
-
 Petroselinum crispum
1.14.20.5 0.016
-
 2-oxoglutarate in presence of 0.02 mM 2S-naringenin Petroselinum crispum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.20.5 soluble
-
 Petroselinum crispum
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.11.9 Fe2+ required Petroselinum crispum
1.14.20.5 Fe2+ required for full activity, cannot be replaced by Mn2+, Co2+ or Ni2+ Petroselinum crispum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.11.9 48000
-
 gel filtration Petroselinum crispum
1.14.20.5 48000
-
 gel filtration Petroselinum crispum

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.9 Petroselinum crispum
-
-
-
1.14.20.5 Petroselinum crispum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.11.9
-
 Petroselinum crispum
1.14.20.5
-
 Petroselinum crispum

Reaction

EC Number Reaction Comment Organism Reaction ID
1.14.11.9 a (2S)-flavan-4-one + 2-oxoglutarate + O2 = a (2R,3R)-dihydroflavonol + succinate + CO2 enzyme catalyzes double-bond formation by direct abstraction of vicinal hydrogen atoms at C-2 and C-3 of the substrate Petroselinum crispum
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.20.5 cell culture
-
 Petroselinum crispum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.11.9 additional information
-
-
 Petroselinum crispum
1.14.20.5 0.0057
-
-
 Petroselinum crispum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.9 (2S)-naringenin + 2-oxoglutarate + O2
-
 Petroselinum crispum (2R,3R)-dihydrokaempferol + succinate + CO2
-
 ?
1.14.11.9 eriodictyol + 2-oxoglutarate + O2
-
 Petroselinum crispum (2R,3R)-dihydroquercetin + succinate + CO2
-
 ?
1.14.11.9 additional information no activity with (+)-dihydrokaempferol and (2R)-naringenin Petroselinum crispum ?
-
 ?
1.14.20.5 (2S)-eriodictyol + 2-oxoglutarate + O2
-
 Petroselinum crispum ? + CO2 + H2O
-
 ?
1.14.20.5 (2S)-naringenin + 2-oxoglutarate + O2
-
 Petroselinum crispum apigenin + succinate + H2O + CO2
-
 ?
1.14.20.5 2-hydroxynaringenin + 2-oxoglutarate + O2
-
 Petroselinum crispum ? + CO2 + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.11.9 dimer 2 * 24000-25000, SDS-PAGE Petroselinum crispum
1.14.20.5 dimer 2 * 24000-25000, SDS-PAGE Petroselinum crispum

Synonyms

EC Number Synonyms Comment Organism
1.14.20.5 flavone synthase I
-
 Petroselinum crispum
1.14.20.5 FNS I
-
 Petroselinum crispum
1.14.20.5 FS I
-
 Petroselinum crispum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.11.9 8.5 8.6
-
 Petroselinum crispum
1.14.20.5 8.5 8.6
-
 Petroselinum crispum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.14.11.9 0.0018
-
 Pyridine 2,4-dicarboxylate
-
 Petroselinum crispum
1.14.20.5 0.0018
-
 Pyridine 2,4-dicarboxylate
-
 Petroselinum crispum
1.14.20.5 0.057
-
 prunin
-
 Petroselinum crispum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.14.20.5 Petroselinum crispum isoelectric focusing, pH gradient 6.6-4.0
-
 4.9