EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.14.12.12 | additional information | - |
three component enzyme system consisting of: component A, i.e. ferredoxinNAP reductase, component B, i.e. terminal oxygenase ISPNAP, and component C, i.e. ferredoxinNAP | Pseudomonas putida |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.12.12 | naphthalene + NADH + O2 | Pseudomonas putida | initial oxidative reaction in bacterial naphthalene catabolism | (1R,2S)-1,2-dihydronaphthalene-1,2-diol + NAD+ | - |
? | |
1.14.12.12 | naphthalene + NADH + O2 | Pseudomonas putida 119 | initial oxidative reaction in bacterial naphthalene catabolism | (1R,2S)-1,2-dihydronaphthalene-1,2-diol + NAD+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.12.12 | Pseudomonas putida | - |
unable to grow on naphthalene | - |
1.14.12.12 | Pseudomonas putida | - |
wild-type grown on naphthalene | - |
1.14.12.12 | Pseudomonas putida 119 | - |
unable to grow on naphthalene | - |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.14.12.12 | naphthalene + NADH + H+ + O2 = (1R,2S)-1,2-dihydronaphthalene-1,2-diol + NAD+ | proposed mechanism | Pseudomonas putida |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.12.12 | naphthalene + NAD(P)H + O2 | - |
Pseudomonas putida | cis-(1R,2S)-1,2-dihydronaphthalene-1,2-diol + NAD(P)+ | only cis-isomer by bacteria, product rapidly autooxidizes to 1,2-naphthoquinone | ? | |
1.14.12.12 | naphthalene + NAD(P)H + O2 | - |
Pseudomonas putida 119 | cis-(1R,2S)-1,2-dihydronaphthalene-1,2-diol + NAD(P)+ | only cis-isomer by bacteria, product rapidly autooxidizes to 1,2-naphthoquinone | ? | |
1.14.12.12 | naphthalene + NADH + O2 | initial oxidative reaction in bacterial naphthalene catabolism | Pseudomonas putida | (1R,2S)-1,2-dihydronaphthalene-1,2-diol + NAD+ | - |
? | |
1.14.12.12 | naphthalene + NADH + O2 | initial oxidative reaction in bacterial naphthalene catabolism | Pseudomonas putida 119 | (1R,2S)-1,2-dihydronaphthalene-1,2-diol + NAD+ | - |
? |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.12.12 | 7.2 | - |
assay at | Pseudomonas putida |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.12.12 | FAD | - |
Pseudomonas putida | |
1.14.12.12 | NADH | - |
Pseudomonas putida | |
1.14.12.12 | NADPH | - |
Pseudomonas putida |