EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.12.3 | expression in Escherichia coli JM109 | Pseudomonas putida |
1.14.12.3 | genes bedC1C2BA, under the control of the tac promoter, subcloned into pLAFR5, successfully conjugated into seven of the Gram-negative cis-1,2-cyclohexanediol-degrading isolates and stably maintained and expressed in three of them, strain CHD1CHD3, the strains grow on cis-1,2-cyclohexanediol as sole carbon source, express an active BDO and oxidise cyclohexene, but none of the three strains is able to grow on cyclohexene as sole carbon source, overview, expression in Escherichia coli strain JM109 | Pseudomonas putida |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.14.12.3 | Fe2+ | required | Pseudomonas putida |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.12.3 | 1-methylcyclohexene + O2 + NADH | Pseudomonas putida | - |
? | - |
? | |
1.14.12.3 | 1-methylcyclohexene + O2 + NADH | Pseudomonas putida ML2 | - |
? | - |
? | |
1.14.12.3 | 2 cyclohexene + 2 O2 + 2 NADH + 2 H+ | Pseudomonas putida | - |
2-cyclohexen-1-ol + cis-1,2-cyclohexanediol + 2 NAD+ + H2O | failure of transformed bacteria to grow on cyclohexene is attributed to the toxicity of metabolic intermediates accumulating from the 2-cyclohexen-1-ol metabolism, cyclohexene metabolism pathways, overview | ? | |
1.14.12.3 | 3-methylcyclohexene + O2 + NADH | Pseudomonas putida | - |
? | - |
? | |
1.14.12.3 | 3-methylcyclohexene + O2 + NADH | Pseudomonas putida ML2 | - |
? | - |
? | |
1.14.12.3 | benzene + O2 + NADH | Pseudomonas putida | - |
cis-cyclohexa-3,5-diene-1,2-diol + NAD+ | - |
? | |
1.14.12.3 | benzene + O2 + NADH | Pseudomonas putida ML2 | - |
cis-cyclohexa-3,5-diene-1,2-diol + NAD+ | - |
? | |
1.14.12.3 | toluene + O2 + NADH | Pseudomonas putida | - |
? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.12.3 | Pseudomonas putida | - |
- |
- |
1.14.12.3 | Pseudomonas putida | - |
gene bedC1C2BA | - |
1.14.12.3 | Pseudomonas putida ML2 | - |
- |
- |
1.14.12.3 | Pseudomonas putida ML2 | - |
gene bedC1C2BA | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.14.12.3 | 0.0049 | - |
recombinant enzyme expressed in Escherichia coli, substrate cyclohexene | Pseudomonas putida |
1.14.12.3 | 0.0077 | - |
recombinant enzyme expressed in Escherichia coli, substrate 1-methylcyclohexene | Pseudomonas putida |
1.14.12.3 | 0.0086 | - |
recombinant enzyme expressed in Escherichia coli, substrate 3-methylcyclohexene | Pseudomonas putida |
1.14.12.3 | 0.0172 | - |
recombinant enzyme expressed in Escherichia coli, substrate toluene | Pseudomonas putida |
1.14.12.3 | 0.0236 | - |
recombinant enzyme expressed in Escherichia coli, substrate benzene | Pseudomonas putida |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.12.3 | 1-methylcyclohexene + O2 + NADH | - |
Pseudomonas putida | ? | - |
? | |
1.14.12.3 | 1-methylcyclohexene + O2 + NADH | - |
Pseudomonas putida ML2 | ? | - |
? | |
1.14.12.3 | 2 cyclohexene + 2 O2 + 2 NADH + 2 H+ | - |
Pseudomonas putida | 2-cyclohexen-1-ol + cis-1,2-cyclohexanediol + 2 NAD+ + H2O | - |
? | |
1.14.12.3 | 2 cyclohexene + 2 O2 + 2 NADH + 2 H+ | - |
Pseudomonas putida | 2-cyclohexen-1-ol + cis-1,2-cyclohexanediol + 2 NAD+ + H2O | failure of transformed bacteria to grow on cyclohexene is attributed to the toxicity of metabolic intermediates accumulating from the 2-cyclohexen-1-ol metabolism, cyclohexene metabolism pathways, overview | ? | |
1.14.12.3 | 3-methylcyclohexene + O2 + NADH | - |
Pseudomonas putida | ? | - |
? | |
1.14.12.3 | 3-methylcyclohexene + O2 + NADH | - |
Pseudomonas putida ML2 | ? | - |
? | |
1.14.12.3 | benzene + NADH + O2 | - |
Pseudomonas putida | cis-cyclohexa-3,5-diene-1,2-diol + NAD+ | the enzyme oxidizes cyclohexene to a mixture of two products, a monohydroxylated 2-cyclohexen-1-ol product and a dihydroxylated cis-1,2-cyclohexanediol | ? | |
1.14.12.3 | benzene + NADH + O2 | - |
Pseudomonas putida ML2 | cis-cyclohexa-3,5-diene-1,2-diol + NAD+ | the enzyme oxidizes cyclohexene to a mixture of two products, a monohydroxylated 2-cyclohexen-1-ol product and a dihydroxylated cis-1,2-cyclohexanediol | ? | |
1.14.12.3 | benzene + O2 + NADH | - |
Pseudomonas putida | cis-cyclohexa-3,5-diene-1,2-diol + NAD+ | - |
? | |
1.14.12.3 | benzene + O2 + NADH | best substrate | Pseudomonas putida | cis-cyclohexa-3,5-diene-1,2-diol + NAD+ | - |
? | |
1.14.12.3 | benzene + O2 + NADH | - |
Pseudomonas putida ML2 | cis-cyclohexa-3,5-diene-1,2-diol + NAD+ | - |
? | |
1.14.12.3 | benzene + O2 + NADH | best substrate | Pseudomonas putida ML2 | cis-cyclohexa-3,5-diene-1,2-diol + NAD+ | - |
? | |
1.14.12.3 | toluene + O2 + NADH | - |
Pseudomonas putida | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.12.3 | BDO | - |
Pseudomonas putida |
1.14.12.3 | benzene dioxygenase | - |
Pseudomonas putida |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.12.3 | 30 | - |
assay at | Pseudomonas putida |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.12.3 | 7.2 | - |
assay at | Pseudomonas putida |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.12.3 | NADH | - |
Pseudomonas putida |