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Literature summary extracted from

  • Wackett, L.P.
    Benzene dioxygenase from Pseudomonas putida, ML2 (NCIB 12190) (1990), Methods Enzymol., 188, 52-60.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.12.3 0.00782
-
 NADH
-
 Pseudomonas putida

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.12.3 Fe2+ the terminal dioxygenase component contains two Fe2+ ions per molecule in addition to two [2Fe-2S] iron-sulfur clusters Pseudomonas putida
1.14.12.3 Iron the terminal dioxygenase component contains two Fe2+ ions per molecule in addition to two [2Fe-2S] iron-sulfur clusters. The ferredoxin component contains one [2Fe-2S] cluster Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.12.3 23500
-
 alpha2, beta2, 2 * 54500 + 2 * 23500, terminal dioxygenase component. 2 * 42000, reductase component. The ferredoxin component has a MW of 12300 Da Pseudomonas putida
1.14.12.3 42000
-
 alpha2, beta2, 2 * 54500 + 2 * 23500, terminal dioxygenase component. 2 * 42000, reductase component. The ferredoxin component has a MW of 12300 Da Pseudomonas putida
1.14.12.3 54500
-
 alpha2, beta2, 2 * 54500 + 2 * 23500, terminal dioxygenase component. 2 * 42000, reductase component. The ferredoxin component has a MW of 12300 Da Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.12.3 benzene + NADH + O2 Pseudomonas putida reaction in benzene catabolism ?
-
 ?
1.14.12.3 benzene + NADH + O2 Pseudomonas putida ML2 reaction in benzene catabolism ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.12.3 Pseudomonas putida
-
-
-
1.14.12.3 Pseudomonas putida ML2
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.12.3
-
 Pseudomonas putida

Storage Stability

EC Number Storage Stability Organism
1.14.12.3 -20°C, stable for 6 months to 1 year Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.12.3 benzene + NADH + O2
-
 Pseudomonas putida cis-cyclohexa-3,5-diene-1,2-diol + NAD+
-
 ?
1.14.12.3 benzene + NADH + O2
-
 Pseudomonas putida ML2 cis-cyclohexa-3,5-diene-1,2-diol + NAD+
-
 ?
1.14.12.3 benzene + NADH + O2 reaction in benzene catabolism Pseudomonas putida ?
-
 ?
1.14.12.3 benzene + NADH + O2 reaction in benzene catabolism Pseudomonas putida ML2 ?
-
 ?
1.14.12.3 trifluoromethylbenzene + NADH + O2
-
 Pseudomonas putida ?
-
 ?
1.14.12.3 trifluoromethylbenzene + NADH + O2
-
 Pseudomonas putida ML2 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.12.3 ? alpha2, beta2, 2 * 54500 + 2 * 23500, terminal dioxygenase component. 2 * 42000, reductase component. The ferredoxin component has a MW of 12300 Da Pseudomonas putida
1.14.12.3 More the benzene dioxygenase comprises three components: 1. flavoprotein, NADH:ferredoxin oxidoreductase, 2. an intermediate electron-transfer protein, or ferredoxin and 3. a terminal dioxygenase Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.12.3 FAD contains FAD Pseudomonas putida
1.14.12.3 Ferredoxin the benzene dioxygenase comprises three components: 1. flavoprotein, NADH:ferredoxin oxidoreductase, 2. an intermediate electron-transfer protein, or ferredoxin and 3. a terminal dioxygenase Pseudomonas putida
1.14.12.3 NADH
-
 Pseudomonas putida