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Literature summary extracted from

  • Schweizer, D.; Markus, A.; Seez, M.; Ruf, H.H.; Lingens, F.
    Purification and some properties of component B of the 4-chlorophenylacetate 3,4-dioxygenase from Pseudomonas species strain CBS 3 (1987), J. Biol. Chem., 262, 9340-9346.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.14.12.9 75% loss of activity after 16 h dialysis Pseudomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.12.9 1,10-phenanthroline 1 mM, 34% inhibition Pseudomonas sp.
1.14.12.9 EDTA 1 mM, 32% inhibition Pseudomonas sp.
1.14.12.9 iodoacetate 0.01 mM, 77% inhibition Pseudomonas sp.
1.14.12.9 KCN 1 mM, 52% inhibition Pseudomonas sp.
1.14.12.9 NEM 1 mM, 38% inhibition Pseudomonas sp.
1.14.12.9 NH4+ inhibition of activity of reductase component Pseudomonas sp.
1.14.12.9 PCMB 0.005 mM, complete inhibition Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.12.9 0.0023
-
ferrocytochrome c
-
Pseudomonas sp.
1.14.12.9 0.0063
-
reduced 2,6-dichlorophenolindophenol
-
Pseudomonas sp.
1.14.12.9 0.032
-
NADH
-
Pseudomonas sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.12.9 Iron reductase component contains an iron-sulfur cluster of the [2Fe-2S]-type, contains 2.1 mol of iron and 1.7 mol of acid-labile sulfide per mol of reductase Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.12.9 35000
-
reductase component of 4-chlorophenylacetate 3,4-dioxygenase, gel filtration Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
1.14.12.9 Pseudomonas sp.
-
-
-
1.14.12.9 Pseudomonas sp. CBS 3
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.14.12.9 reductase component loses activity in presence of molecular oxygen Pseudomonas sp.

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.12.9 reductase component Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.12.9 30
-
reductase component of of 4-chlorophenylacetate 3,4-dioxygenase Pseudomonas sp.

Storage Stability

EC Number Storage Stability Organism
1.14.12.9 4°C, 20 mM potassium phosphate buffer, pH 6.0-7.5, 10 days, 50% loss of activity Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.12.9 4-chlorophenylacetate + ferrocytochrome c + H+ + O2
-
Pseudomonas sp. 3,4-dihydroxyphenylacetate + chloride + ferricytochrome c
-
r
1.14.12.9 4-chlorophenylacetate + ferrocytochrome c + H+ + O2
-
Pseudomonas sp. CBS 3 3,4-dihydroxyphenylacetate + chloride + ferricytochrome c
-
r
1.14.12.9 4-chlorophenylacetate + NADH + O2 + H2O
-
Pseudomonas sp. 3,4-dihydroxyphenylacetate + Cl- + NAD(P)+
-
?
1.14.12.9 4-chlorophenylacetate + NADH + O2 + H2O
-
Pseudomonas sp. CBS 3 3,4-dihydroxyphenylacetate + Cl- + NAD(P)+
-
?
1.14.12.9 4-chlorophenylacetate + reduced 2,6-dichlorophenolindophenol + O2
-
Pseudomonas sp. 3,4-dihydroxyphenylacetate + chloride + oxidized 2,6-dichlorophenolindophenol
-
r
1.14.12.9 4-chlorophenylacetate + reduced 2,6-dichlorophenolindophenol + O2
-
Pseudomonas sp. CBS 3 3,4-dihydroxyphenylacetate + chloride + oxidized 2,6-dichlorophenolindophenol
-
r
1.14.12.9 additional information the reductase component reduces several redox compounds: 2,6-dichlorophenylindophenol, potassium hexacyanoferrate III, cytochrome c, methylene blue and nitro blue tetrazolium Pseudomonas sp. ?
-
?
1.14.12.9 additional information the reductase component reduces several redox compounds: 2,6-dichlorophenylindophenol, potassium hexacyanoferrate III, cytochrome c, methylene blue and nitro blue tetrazolium Pseudomonas sp. CBS 3 ?
-
?

Subunits

EC Number Subunits Comment Organism
1.14.12.9 monomer 1 * 35000, reductase component of of 4-chlorophenylacetate 3,4-dioxygenase, SDS-PAGE Pseudomonas sp.
1.14.12.9 More the enzyme consists of two components, a monomeric reductase and a trimeric dioxygenase Pseudomonas sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.14.12.9 45
-
3 min, 50% loss of activity Pseudomonas sp.
1.14.12.9 55
-
90-100% loss of activity Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.12.9 7 7.5
-
Pseudomonas sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.14.12.9 6 7.5 4°C, 10 days, 50% loss of activity Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.12.9 FMN flavoprotein, contains 1 mol of FMN per mol of reductase, no increase of activity by addition of exogenous FMN Pseudomonas sp.
1.14.12.9 NADH
-
Pseudomonas sp.
1.14.12.9 NADPH 30% as effective as NADH Pseudomonas sp.