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Literature summary extracted from
- The bacterial oxidation of nicotine. VII. Partial purification and properties of 2,6-dihydroxypyridine oxidase (1972), J. Biol. Chem., 247, 7622-7627.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.13.10 | CO | 35% activation | Paenarthrobacter nicotinovorans |  |
| 1.14.13.10 | EDTA | 38% activation at 0.05 mM | Paenarthrobacter nicotinovorans | |
| 1.14.13.10 | KCN | 18% activation at 0.5 mM | Paenarthrobacter nicotinovorans | |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.13.10 | Cu2+ | 50% inhibition at 0.017 mM | Paenarthrobacter nicotinovorans | |
| 1.14.13.10 | Hg2+ | 50% inhibition at 0.005 mM | Paenarthrobacter nicotinovorans | |
| 1.14.13.10 | NaN3 | 50% inhibition at 2 mM | Paenarthrobacter nicotinovorans | |
| 1.14.13.10 | p-chloromercuribenzoate | 50% inhibition at 0.01 mM | Paenarthrobacter nicotinovorans | |
| 1.14.13.10 | Zn2+ | 50% inhibition at 0.7 mM | Paenarthrobacter nicotinovorans | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.14.13.10 | soluble | - |
Paenarthrobacter nicotinovorans | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.10 | 89000 | - |
sucrose density gradient centrifugation | Paenarthrobacter nicotinovorans |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.13.10 | 2,6-dihydroxypyridine + NADH + O2 | Paenarthrobacter nicotinovorans | - |
2,3,6-trihydroxypyridine + NAD+ + H2O | - |
? | |
Organic Solvent Stability
| EC Number | Organic Solvent | Comment | Organism |
|---|---|---|---|
| 1.14.13.10 | Acetone | complete loss of activity | Paenarthrobacter nicotinovorans |
| 1.14.13.10 | Ethanol | stabilizes during preparative manipulations | Paenarthrobacter nicotinovorans |
| 1.14.13.10 | Glycerol | stabilizes during preparative manipulations | Paenarthrobacter nicotinovorans |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.13.10 | Paenarthrobacter nicotinovorans | - |
formerly Arthrobacter oxidans | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.13.10 | partial | Paenarthrobacter nicotinovorans |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.10 | 2.16 | - |
- |
Paenarthrobacter nicotinovorans |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.13.10 | 2,6-dihydroxypyridine + NADH + electron acceptor | methylene blue and 2,6-dichlorophenol-indophenol can act as alternative electron acceptors | Paenarthrobacter nicotinovorans | 2,3,6-trihydroxypyridine + NAD+ + reduced electron acceptor | - |
? | |
| 1.14.13.10 | 2,6-dihydroxypyridine + NADH + O2 | - |
Paenarthrobacter nicotinovorans | 2,3,6-trihydroxypyridine + NAD+ + H2O | - |
? | |
| 1.14.13.10 | 2,6-dihydroxypyridine + NADH + O2 | - |
Paenarthrobacter nicotinovorans | 2,3,6-trihydroxypyridine + NAD+ + H2O | pyridine ring cleavage follows in a second reaction | ? | |
| 1.14.13.10 | additional information | 2-hydroxypyridine, 3-hydroxypyridine, 4-hydroxypyridine, 2,5-dihydroxypyridine, 2,6-dipicolinic acid, p-hydroxybenzoic acid, nicotinic acid and nicotine are no substrates for this enzyme | Paenarthrobacter nicotinovorans | ? | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.10 | 30 | - |
- |
Paenarthrobacter nicotinovorans |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.10 | 15 | - |
30% loss of activity in 10 min | Paenarthrobacter nicotinovorans |
| 1.14.13.10 | 30 | - |
complete loss of activity in 10 min | Paenarthrobacter nicotinovorans |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.10 | 8 | - |
- |
Paenarthrobacter nicotinovorans |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.10 | 7 | - |
most stable in 50 mM potassium phosphate buffer | Paenarthrobacter nicotinovorans |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.13.10 | FAD | - |
Paenarthrobacter nicotinovorans | |
| 1.14.13.10 | NADH | - |
Paenarthrobacter nicotinovorans | |
| 1.14.13.10 | NADPH | less effective than NADH | Paenarthrobacter nicotinovorans | |
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Release 2023.1 (January 2023)
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