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Literature summary extracted from

  • Forkmann, G.; Heller, W.; Grisebach, H.
    Anthocyanin biosynthesis in flowers of Matthiola incana flavanone 3- and flavonoid 3'-hydroxylases (1980), Z. Naturforsch. C, 35, 691-695.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.11.9 ascorbate required Matthiola incana

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.11.9 diethyldicarbonate 0.5 mM, 15% inhibition; slight Matthiola incana
1.14.11.9 diethyldithiocarbamate 2 mM, complete inhibition Matthiola incana
1.14.11.9 EDTA 1 mM, 75% inhibition Matthiola incana
1.14.11.9 KCN 5 mM, complete inhibition Matthiola incana
1.14.11.9 p-chloromercuribenzoate 0.1 mM, 5% inhibition Matthiola incana
1.14.14.82 diethyldicarbonate
-
 Matthiola incana
1.14.14.82 EDTA
-
 Matthiola incana
1.14.14.82 KCN
-
 Matthiola incana
1.14.14.82 p-chloromercuribenzoate partial Matthiola incana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.11.9 soluble
-
 Matthiola incana
-
-
1.14.14.82 microsome
-
 Matthiola incana
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.11.9 Fe2+ required Matthiola incana

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.9 Matthiola incana
-
-
-
1.14.14.82 Matthiola incana
-
 enzyme present only in lines of organism with wild-type allele b+
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.11.9 flower
-
 Matthiola incana
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.11.9 additional information
-
-
 Matthiola incana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.9 (2S)-naringenin + 2-oxoglutarate + O2
-
 Matthiola incana (2R,3R)-dihydrokaempferol + succinate + CO2
-
 ?
1.14.11.9 eriodictyol + 2-oxoglutarate + O2
-
 Matthiola incana (2R,3R)-dihydroquercetin + succinate + CO2
-
 ?
1.14.14.82 dihydrokaempferol + [reduced NADPH-hemoprotein reductase] + O2
-
 Matthiola incana dihydroquercetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.82 additional information not: 4-coumarate, 4-coumaroyl-CoA Matthiola incana ?
-
 ?
1.14.14.82 naringenin + [reduced NADPH-hemoprotein reductase] + O2
-
 Matthiola incana eriodictyol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.14.82 7.2
-
 naringenin Matthiola incana