EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.13.22 | expression of His-tagged enzyme in Escherichia coli and Saccharomyces cerevisiae | Acinetobacter sp. |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.14.13.22 | additional information | His-tagged recombinant enzyme expressed in Escherichia coli is, despite the tag, similar to the wild-type enzyme, whereas the recombinant His-tagged enzyme expressed in yeast is posttranslationally modified, Escherichia coli is the preferred expression system | Acinetobacter sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.14.13.22 | 60800 | - |
wild-type, electrospray mass spectrometry | Acinetobacter sp. |
1.14.13.22 | 61620 | - |
recombinant His-tagged enzyme expressed in E. coli | Acinetobacter sp. |
1.14.13.22 | 61670 | - |
recombinant His-tagged enzyme expressed in yeast | Acinetobacter sp. |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.13.22 | Acinetobacter sp. | Q9R2F5 | NCIB 9871 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.13.22 | native enzyme and recombinant His-tagged enzyme from Escherichia coli and Saccharomyces cerevisiae | Acinetobacter sp. |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.14.13.22 | 14 | - |
purified recombinant His-tagged enzyme expressed in yeast | Acinetobacter sp. |
1.14.13.22 | 15 | - |
purified native enzyme | Acinetobacter sp. |
1.14.13.22 | 19.9 | - |
purified recombinant His-tagged enzyme expressed in E. coli | Acinetobacter sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.13.22 | cyclohexanone + NADPH + O2 | - |
Acinetobacter sp. | 6-hexanolide + NADP+ + H2O | product: epsilon-caprolactone i.e. 1-oxa-2-oxocycloheptane | ? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.13.22 | CMO | - |
Acinetobacter sp. |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.22 | FAD | - |
Acinetobacter sp. | |
1.14.13.22 | NADPH | - |
Acinetobacter sp. |