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Literature summary extracted from

  • Trower, M.K.; Buckland, R.M.; Griffin, M.
    Characterization of a cyclohexane hydrolase and a cyclohexanone mono-oxygenase from a cyclohexane-degrading Xanthobacter sp. (1985), Biochem. Soc. Trans., 13, 463-464.
No PubMed abstract available

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.13.22 cytoplasm
-
 Pseudomonas sp. 5737
-
1.14.13.22 cytoplasm
-
 Xanthobacter sp. 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.13.22 51000
-
 1 * 51000, SDS-PAGE Xanthobacter sp.

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.22 Pseudomonas sp.
-
-
-
1.14.13.22 Xanthobacter sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.22
-
 Xanthobacter sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.13.22 2.1
-
-
 Xanthobacter sp.

Storage Stability

EC Number Storage Stability Organism
1.14.13.22 -20°C, stable Xanthobacter sp.
1.14.13.22 4°C, in presence of cyclohexane or 0.1 mM NADPH Xanthobacter sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.22 1-phenyl-2-propanone + NADPH + O2
-
 Pseudomonas sp. ?
-
 ?
1.14.13.22 1-phenyl-2-propanone + NADPH + O2
-
 Xanthobacter sp. ?
-
 ?
1.14.13.22 2-phenyl-1-ethanal + NADPH + O2
-
 Pseudomonas sp. ?
-
 ?
1.14.13.22 2-phenyl-1-ethanal + NADPH + O2
-
 Xanthobacter sp. ?
-
 ?
1.14.13.22 cyclohexanone + NADPH + O2
-
 Xanthobacter sp. 6-hexanolide + NADP+ + H2O
-
 ?
1.14.13.22 additional information no activity towards straight-chain alkanones Pseudomonas sp. ?
-
 ?
1.14.13.22 additional information no activity towards straight-chain alkanones Xanthobacter sp. ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.13.22 monomer 1 * 51000, SDS-PAGE Xanthobacter sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.13.22 30
-
 assay at Pseudomonas sp.
1.14.13.22 30
-
 assay at Xanthobacter sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.13.22 8.8
-
-
 Xanthobacter sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.22 FMN 1 FMN molecule per molecule of protein Xanthobacter sp.
1.14.13.22 NADPH absolutely specific for NADPH as electron donor Pseudomonas sp.
1.14.13.22 NADPH absolutely specific for NADPH as electron donor Xanthobacter sp.