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Literature summary extracted from
- Studies on m-hydroxybenzoate 4-hydroxylase from Comamonas testosteroni I. Purification and characterization (1997), Res. Commun. Biochem. Cell Mol. Biol., 1, 304-322.
No PubMed abstract available
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.13.23 | 0.03 | - |
NADPH | - |
Comamonas testosteroni |  |
| 1.14.13.23 | 0.03 | - |
m-Hydroxybenzoate | - |
Comamonas testosteroni | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.23 | 71000 | - |
x * 71000, SDS-PAGE | Comamonas testosteroni |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.13.23 | 3-hydroxybenzoate + [reduced NADPH-hemoprotein reductase] + O2 | Comamonas testosteroni | - |
3,4-dihydroxybenzoate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.13.23 | Comamonas testosteroni | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.13.23 | - |
Comamonas testosteroni |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.23 | 5.17 | - |
- |
Comamonas testosteroni |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.13.23 | 2,3-dihydroxybenzoate + NADPH + O2 | - |
Comamonas testosteroni | ? | - |
? | |
| 1.14.13.23 | 3-hydroxybenzoate + [reduced NADPH-hemoprotein reductase] + O2 | - |
Comamonas testosteroni | 3,4-dihydroxybenzoate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.13.23 | gentisate + NADPH + O2 | - |
Comamonas testosteroni | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.14.13.23 | ? | x * 71000, SDS-PAGE | Comamonas testosteroni |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.13.23 | FAD | flavoprotein | Comamonas testosteroni | |
| 1.14.13.23 | NADPH | specific requirement for NADPH | Comamonas testosteroni | |
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