Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Michalover, J.M.; Ribbons, D.W.
    3-Hydroxybenzoate 4-hydroxylase from Pseudomonas testosteroni (1973), Biochem. Biophys. Res. Commun., 55, 888-896.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.23 0.02
-
NADPH + 2-fluoro-5-hydroxybenzoate Comamonas testosteroni
1.14.13.23 0.03
-
3-hydroxybenzoate cosubstrate NADPH Comamonas testosteroni
1.14.13.23 0.04
-
3,5-Dihydroxybenzoate cosubstrate NADPH Comamonas testosteroni
1.14.13.23 0.05
-
NADPH cosubstrates 3-hydroxyanthranilate or 3,5-dihydroxybenzoate Comamonas testosteroni
1.14.13.23 0.06
-
2,3-Dihydroxybenzoate cosubstrate NADPH Comamonas testosteroni
1.14.13.23 0.07
-
NADPH cosubstrates 3-hydroxybenzoate or 2,3-dihydroxybenzoate Comamonas testosteroni
1.14.13.23 0.12
-
4-Fluoro-3-hydroxybenzoate cosubstrate NADPH Comamonas testosteroni
1.14.13.23 0.14
-
NADPH + 2,5-dihydroxybenzoate Comamonas testosteroni
1.14.13.23 0.15
-
NADPH + 4-fluoro-3-hydroxybenzoate Comamonas testosteroni
1.14.13.23 0.5
-
2,5-Dihydroxybenzoate cosubstrate NADPH Comamonas testosteroni
1.14.13.23 3
-
NADH cosubstrate 3-hydroxybenzoate Comamonas testosteroni

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.13.23 145000
-
sedimentation equilibrium centrifugation Comamonas testosteroni

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.23 3-hydroxybenzoate + [reduced NADPH-hemoprotein reductase] + O2 Comamonas testosteroni
-
3,4-dihydroxybenzoate + [oxidized NADPH-hemoprotein reductase] + H2O
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.23 Comamonas testosteroni
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.23
-
Comamonas testosteroni

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.13.23 11.8
-
-
Comamonas testosteroni

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.23 2,3-dihydroxybenzoate + NADPH + O2
-
Comamonas testosteroni ?
-
?
1.14.13.23 2,5-dihydroxybenzoate + NADPH + O2
-
Comamonas testosteroni ?
-
?
1.14.13.23 2-fluoro-5-hydroxybenzoate + NADPH + O2
-
Comamonas testosteroni ?
-
?
1.14.13.23 3,5-dihydroxybenzoate + NADPH + O2
-
Comamonas testosteroni ?
-
?
1.14.13.23 3-hydroxyanthranilate + NADPH + O2
-
Comamonas testosteroni ?
-
?
1.14.13.23 3-hydroxybenzoate + NADH + O2
-
Comamonas testosteroni 3,4-dihydroxybenzoate + NAD+ + H2O
-
?
1.14.13.23 3-hydroxybenzoate + [reduced NADPH-hemoprotein reductase] + O2
-
Comamonas testosteroni 3,4-dihydroxybenzoate + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
1.14.13.23 4-fluoro-3-hydroxybenzoate + NADPH + O2
-
Comamonas testosteroni ?
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.13.23 30
-
assay at Comamonas testosteroni

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.23 FAD flavoprotein Comamonas testosteroni
1.14.13.23 NADH poor substitute for NADPH Comamonas testosteroni
1.14.13.23 NADPH
-
Comamonas testosteroni