Literature summary extracted from
Lloyd, J.S.; Bhambra, A.; Murrell, J.C.; Dalton, H.
Inactivation of the regulatory protein B of soluble methane monooxygenase from Methylococcus capsulatus (Bath) by proteolysis can be overcome by a Gly to Gln modification (1997), Eur. J. Biochem., 248, 72-79.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
1.14.13.25 |
expression of component protein B of sMMO as glutathione-S-transferase fusion protein in Escherichia coli |
Methylococcus capsulatus |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
1.14.13.25 |
G13Q |
enhanced temperature stability compared to wild-type, site-directed mutagenesis |
Methylococcus capsulatus |
1.14.13.25 |
G13Q |
sMMO, alteration of a cleavage site in component protein B |
Methylococcus capsulatus |
1.14.13.25 |
additional information |
native parallel occurence of full length and 2 N-terminal truncated forms of regulatory component protein B of sMMO, truncated forms are inactive |
Methylococcus capsulatus |
Localization
EC Number |
Localization |
Comment |
Organism |
GeneOntology No. |
Textmining |
---|
1.14.13.25 |
cytoplasm |
cytoplasmatic, soluble enzyme form termed sMMO |
Methylococcus capsulatus |
5737 |
- |
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
1.14.13.25 |
15850 |
- |
component protein B of sMMO, mass spectrometry |
Methylococcus capsulatus |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.14.13.25 |
Methylococcus capsulatus |
- |
enzyme form sMMO |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
1.14.13.25 |
recombinant component protein B of sMMO as glutathione-S-transferase fusion protein from Escherichia coli |
Methylococcus capsulatus |
Specific Activity [micromol/min/mg]
EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
---|
1.14.13.25 |
additional information |
- |
recombinant shows higher activity than the wild-type |
Methylococcus capsulatus |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
1.14.13.25 |
methane + NAD(P)H + O2 |
- |
Methylococcus capsulatus |
methanol + NAD(P)+ + H2O |
- |
? |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
1.14.13.25 |
MMO |
- |
Methylococcus capsulatus |
1.14.13.25 |
pMMO |
particulate, membrane-bound enzyme form |
Methylococcus capsulatus |
1.14.13.25 |
sMMO |
soluble, cytoplasmic enzyme form |
Methylococcus capsulatus |
Cofactor
EC Number |
Cofactor |
Comment |
Organism |
Structure |
---|
1.14.13.25 |
NADH |
- |
Methylococcus capsulatus |
|