Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Zahn, J.A.; DiSpirito, A.A.
    Membrane-associated methane monooxygenase from Methylococcus capsulatus (Bath) (1996), J. Bacteriol., 178, 1018-1029.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.18.3 Cu2+ pMMO, optimal at 0.3 mM Methylococcus capsulatus
1.14.18.3 Fe3+ pMMO, optimal at 5.0 mM Methylococcus capsulatus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.18.3 2-Heptyl-4-hydroxyquinoline-N-oxide pMMO, at 0.05 mM Methylococcus capsulatus
1.14.18.3 Acetylene pMMO Methylococcus capsulatus
1.14.18.3 Cu+ pMMO Methylococcus capsulatus
1.14.18.3 cysteine pMMO Methylococcus capsulatus
1.14.18.3 Fe2+ slightly, membrane-bound enzyme form Methylococcus capsulatus
1.14.18.3 KCN pMMO Methylococcus capsulatus
1.14.18.3 Myxothiazol pMMO, suicide substrate Methylococcus capsulatus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.18.3 cytoplasm cytoplasmatic, soluble enzyme form termed sMMO Methylococcus capsulatus 5737
-
1.14.18.3 membrane membrane-bound particulate enzyme form termed pMMO Methylococcus capsulatus 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.18.3 Cu2+ 14.5 atoms per molecule of enzyme pMMO, type II copper centre Methylococcus capsulatus
1.14.18.3 Iron 2.5 atoms per enzyme molecule of pMMO Methylococcus capsulatus
1.14.18.3 Mn2+ pMMO, low content Methylococcus capsulatus
1.14.18.3 Mo2+ pMMO, low content Methylococcus capsulatus
1.14.18.3 additional information copper-induced iron-uptake Methylococcus capsulatus
1.14.18.3 Zn2+ pMMO Methylococcus capsulatus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.18.3 25000
-
 1 * 47000 + 1 * 27000 + 1 * 25000, pMMO, mass spectrometry and SDS-PAGE Methylococcus capsulatus
1.14.18.3 27000
-
 1 * 47000 + 1 * 27000 + 1 * 25000, pMMO, mass spectrometry and SDS-PAGE Methylococcus capsulatus
1.14.18.3 47000
-
 1 * 47000 + 1 * 27000 + 1 * 25000, pMMO, mass spectrometry and SDS-PAGE Methylococcus capsulatus
1.14.18.3 99000
-
 pMMO, mass spectrometry Methylococcus capsulatus

Organism

EC Number Organism UniProt Comment Textmining
1.14.18.3 Methylococcus capsulatus
-
 Bath
-
1.14.18.3 Methylococcus capsulatus
-
 enzyme form pMMO is copper-inducible
-
1.14.18.3 Methylococcus capsulatus Bath
-
 Bath
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.18.3 pMMO Methylococcus capsulatus

Reaction

EC Number Reaction Comment Organism Reaction ID
1.14.18.3 methane + quinol + O2 = methanol + quinone + H2O mechanism of pMMO Methylococcus capsulatus
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.18.3 0.011
-
 purified enzyme form pMMO Methylococcus capsulatus
1.14.18.3 0.0605
-
 whole cells, substrate formate Methylococcus capsulatus

Storage Stability

EC Number Storage Stability Organism
1.14.18.3 4°C, reducing argon or nitrogen atmosphere, no loss of activity after 1 week Methylococcus capsulatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.18.3 methane + reduced acceptor + H* + O2
-
 Methylococcus capsulatus methanol + acceptor + H2O
-
 ?
1.14.18.3 methane + reduced acceptor + H* + O2
-
 Methylococcus capsulatus Bath methanol + acceptor + H2O
-
 ?
1.14.18.3 propene + reduced acceptor + H+ + O2 enzyme form sMMO Methylococcus capsulatus 1,2-epoxypropane + acceptor + H2O
-
 ?
1.14.18.3 propene + reduced acceptor + H+ + O2 enzyme form sMMO Methylococcus capsulatus Bath 1,2-epoxypropane + acceptor + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.18.3 More pMMO subunit A has acetylene binding ability Methylococcus capsulatus
1.14.18.3 trimer 1 * 47000 + 1 * 27000 + 1 * 25000, pMMO, mass spectrometry and SDS-PAGE Methylococcus capsulatus

Synonyms

EC Number Synonyms Comment Organism
1.14.18.3 MMO
-
 Methylococcus capsulatus
1.14.18.3 pMMO particulate, membrane-bound enzyme form Methylococcus capsulatus
1.14.18.3 sMMO soluble, cytoplasmic enzyme form Methylococcus capsulatus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.18.3 NADH
-
 Methylococcus capsulatus