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Literature summary extracted from

  • Colby, J.; Dalton, H.
    Characterization of the second prosthetic group of the flavoenzyme NADH-acceptor reductase (component C) of the methane mono-oxygenase from Methylococcus capsulatus (Bath) (1979), Biochem. J., 177, 903-908.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
1.14.13.25 5 mM thioglycollate, 5 mM dithiothreitol, 5 mM NADH stabilize component C at 0°C Methylococcus capsulatus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.13.25 8-hydroxyquinoline compound C not inhibited Methylococcus capsulatus
1.14.13.25 Acetylene component C not inhibited Methylococcus capsulatus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.13.25 Iron 1 mol of [2Fe-2S(S-Cys)4]centre per mol protein Methylococcus capsulatus
1.14.13.25 Iron 2 g-atom iron Methylococcus capsulatus
1.14.13.25 Iron characterization of [Fe2-S2] redox centre of component C Methylococcus capsulatus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.13.25 additional information
-
 enzyme system consists of 3 protein components A, B, C Methylococcus capsulatus

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.25 Methylococcus capsulatus
-
 Bath
-
1.14.13.25 Methylococcus capsulatus Bath
-
 Bath
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.25 component C Methylococcus capsulatus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.13.25 6
-
 purified protein C Methylococcus capsulatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.25 ethene + NAD(P)H + O2
-
 Methylococcus capsulatus epoxyethane + NAD(P)+ + H2O
-
 ?
1.14.13.25 ethene + NAD(P)H + O2
-
 Methylococcus capsulatus Bath epoxyethane + NAD(P)+ + H2O
-
 ?
1.14.13.25 methane + NAD(P)H + O2
-
 Methylococcus capsulatus methanol + NAD(P)+ + H2O
-
 ?
1.14.13.25 methane + NAD(P)H + O2
-
 Methylococcus capsulatus Bath methanol + NAD(P)+ + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.13.25 More enzyme system consists of 3 protein components A, B, C Methylococcus capsulatus
1.14.13.25 More component A is a hydroxylase Methylococcus capsulatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.13.25 45
-
 assay at Methylococcus capsulatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.13.25 8.5 9 component C Methylococcus capsulatus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.25 FAD protein C, reductase component: contains 1 mol FAD per mol protein Methylococcus capsulatus
1.14.13.25 NADH
-
 Methylococcus capsulatus